基于 Monostyryl Boradiazaindacene（BODIPY）的镧系元素-free 比色和荧光探针，用于顺序感测铜（II）离子和二吡啶甲酸，作为细菌芽孢的生物标志物。
A Monostyryl Boradiazaindacene (BODIPY)-based lanthanide-free colorimetric and fluorogenic probe for sequential sensing of copper (II) ions and dipicolinic acid as a biomarker of bacterial endospores.
机构信息
Department of Bioengineering, Faculty of Engineering and Architecture, Konya Food and Agriculture University, 42080 Konya, Turkey.
Research and Development Center for Diagnostic Kits (KIT-ARGEM), Konya Food and Agriculture University, 42080 Konya, Turkey; Institute of Science, Biotechnology Graduate Program, Konya Food and Agriculture University, Konya, 42080, Turkey.
出版信息
J Hazard Mater. 2019 Sep 5;377:299-304. doi: 10.1016/j.jhazmat.2019.05.108. Epub 2019 May 31.
A new catechol-substituted monostyryl boradiazaindacene (BODIPY)-based lanthanide-free colorimetric and fluorogenic probe was developed for the sequential detection of Cu ions and dipicolinic acid (DPA), a distinctive biomarker of bacterial endospores, with high sensitivity and selectivity. In the presence of Cu ions, the blue solution of the probe changes to cyan and the fluorescence is quenched, however, the cyan color changes to blue immediately and the fluorescence is restored on contact with DPA, resulting from competitive binding of DPA that interact with Cu ions. A practical application by using Geobacillus stearothermophilus spores was further studied and as low as 1.0 x 10 spores were detected.
一种新型的儿茶酚取代的单苯乙烯基硼二吡咯亚甲基（BODIPY）-基镧系元素自由比色和荧光探针被开发出来，用于顺序检测 Cu 离子和二吡啶甲酸（DPA），DPA 是细菌芽孢的特征生物标志物，具有高灵敏度和选择性。在 Cu 离子存在下，探针的蓝色溶液变为青色，荧光被猝灭，但与 DPA 接触后，青色颜色立即变为蓝色，荧光恢复，这是由于 DPA 与 Cu 离子竞争结合所致。进一步研究了使用嗜热脂肪芽孢杆菌芽孢的实际应用，可检测到低至 1.0×10 个芽孢。
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