Yang Shangye, Huang Tinglin, Zhang Haihan, Guo Honghong, Hu Ruzhu, Lin Zishen, Li Yanqing, Cheng Ya
Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
Environ Res. 2023 Nov 1;236(Pt 2):116830. doi: 10.1016/j.envres.2023.116830. Epub 2023 Aug 4.
Nitrogen pollution poses a severe threat to aquatic ecosystems and human health. This study investigated the use of water lifting aerators for in situ nitrogen reduction in a drinking water reservoir. The reservoir was thoroughly mixed and oxygenated after using water-lifting aerators for 42 days. The average total nitrogen concentration, nitrate nitrogen, and ammonium nitrogen-in all water layers-decreased significantly (P < 0.01), with a reduction efficiency of 35 ± 3%, 34 ± 2%, and 70 ± 6%, respectively. Other pollutants, including organic matter, phosphorus, iron, and manganese, were also effectively removed. Quantitative polymerase chain reactions indicated that bacterial nirS gene abundance was enhanced 26.34-fold. High-throughput sequencing, phylogenetic tree, and network analysis suggested that core indigenous nirS-type denitrifying bacteria, such as Dechloromonas, Simplicispira, Thauera, and Azospira, played vital roles in nitrogen and other pollutant removal processes. Furthermore, structural equation modeling revealed that nitrogen removal responded positively to WT, DO, and nirS gene abundance. Our findings provide a promising strategy for nitrogen removal in oligotrophic drinking water reservoirs with carbon deficiencies.
氮污染对水生生态系统和人类健康构成严重威胁。本研究调查了在饮用水水库中使用提水曝气器进行原位脱氮的情况。使用提水曝气器42天后，水库得到了充分混合和增氧。所有水层中的平均总氮浓度、硝酸盐氮和铵态氮均显著降低（P < 0.01），去除效率分别为35±3%、34±2%和70±6%。包括有机物、磷、铁和锰在内的其他污染物也被有效去除。定量聚合酶链反应表明细菌nirS基因丰度提高了26.34倍。高通量测序、系统发育树和网络分析表明，核心本土nirS型反硝化细菌，如脱氯单胞菌属、简单螺旋菌属、陶厄氏菌属和固氮螺菌属，在氮和其他污染物的去除过程中发挥了重要作用。此外，结构方程模型显示脱氮对水温、溶解氧和nirS基因丰度有正向响应。我们的研究结果为贫营养且碳缺乏的饮用水水库脱氮提供了一种有前景的策略。
