Ghanbari Elham, Khazaei Mozafar, Mehdipour Ahmad, Khoshfeterat Alibaradar, Niknafs Behrooz
Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Fertility and Infertility Research Centre, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Cell J. 2023 Jul 25;25(7):483-495. doi: 10.22074/cellj.2023.1986179.1204.
The use of biocompatible scaffolds with appropriate characteristics to treat large bone defects has attracted significant attention. The main objective of the current study is to fabricate a 3D nanocomposite structure that contains green synthesized magnesium oxide nanoparticles (MgONPs) and bacterial cellulose (BC) nanofibres, as a bioscaffold for bone regeneration.
In this experimental study, sinensis extract was used as the green method to synthesize MgONPs. The synthesized hydrogels were evaluated for their porosity, morphology, degradation rate, mechanical features, cell attachment, and cytocompatibility. Osteogenic differentiation was assessed by alkaline phosphatase (ALP) activity, real-time reverse transcription-polymerase chain reaction (RT-PCR), and alizarin red staining.
MgONPs significantly increased both mechanical strength (P=0.009) and porosity (P=0.01) of the BC hydrogels. Human MG-63 osteoblast proliferation significantly increased in the MgONP-BC group compared to the pure BC group (P=0.003). Expression rates of both the ALP (P=0.001) and osteocalcin (OCN) genes were significantly enhanced in cells seeded on the MgONP-incorporated BC. MG-63 cells had significantly greater calcium deposition and ALP activity (P=0.002) on the MgONP-BC scaffold compared to the BC at day 21.
The MgONP-BC scaffold can promote the osteogenic activity of osteoblast-like cells, which indicates its therapeutic potential for bone tissue regeneration.
使用具有适当特性的生物相容性支架治疗大的骨缺损已引起了广泛关注。本研究的主要目的是制备一种包含绿色合成的氧化镁纳米颗粒（MgONPs）和细菌纤维素（BC）纳米纤维的三维纳米复合结构，作为骨再生的生物支架。
在本实验研究中，使用中华猕猴桃提取物作为绿色方法合成MgONPs。对合成的水凝胶的孔隙率、形态、降解率、机械性能、细胞附着和细胞相容性进行评估。通过碱性磷酸酶（ALP）活性、实时逆转录聚合酶链反应（RT-PCR）和茜素红染色评估成骨分化。
MgONPs显著提高了BC水凝胶的机械强度（P = 0.009）和孔隙率（P = 0.01）。与纯BC组相比，MgONP-BC组人MG-63成骨细胞增殖显著增加（P = 0.003）。接种在含MgONP的BC上的细胞中，ALP（P = 0.001）和骨钙素（OCN）基因的表达率均显著提高。在第21天，与BC相比，MG-63细胞在MgONP-BC支架上具有显著更高的钙沉积和ALP活性（P = 0.002）。
MgONP-BC支架可促进成骨样细胞的成骨活性，这表明其在骨组织再生方面具有治疗潜力。
