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细菌纤维素改性聚羟基脂肪酸酯支架促进小鼠临界尺寸颅骨缺损的骨形成

Bacterial Cellulose-Modified Polyhydroxyalkanoates Scaffolds Promotes Bone Formation in Critical Size Calvarial Defects in Mice.

作者信息

Codreanu Ada, Balta Cornel, Herman Hildegard, Cotoraci Coralia, Mihali Ciprian Valentin, Zurbau Nicoleta, Zaharia Catalin, Rapa Maria, Stanescu Paul, Radu Ionut-Cristian, Vasile Eugeniu, Lupu George, Galateanu Bianca, Hermenean Anca

机构信息

Faculty of Medicine, Vasile Goldis Western University of Arad, 94-96 Revolutiei Avenue, 310025 Arad, Romania.

"Aurel Ardelean" Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu Street, 310414 Arad, Romania.

出版信息

Materials (Basel). 2020 Mar 21;13(6):1433. doi: 10.3390/ma13061433.

DOI:10.3390/ma13061433
PMID:32245214
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7142421/
Abstract

Bone regeneration is a claim challenge in addressing bone defects with large tissue deficits, that involves bone grafts to support the activity. In vitro biocompatibility of the bacterial cellulose-modified polyhydroxyalkanoates (PHB/BC) scaffolds and its osteogenic potential in critical-size mouse calvaria defects had been investigated. Bone promotion and mineralization were analyzed by biochemistry, histology/histomorphometry, X-ray analysis and immunofluorescence for highlighting osteogenesis markers. In summary, our results showed that PHB/BC scaffolds are able to support 3T3-L1 preadipocytes proliferation and had a positive effect on in vivo osteoblast differentiation, consequently inducing new bone formation after 20 weeks post-implantation. Thus, the newly developed PHB/BC scaffolds could turn out to be suitable biomaterials for the bone tissue engineering purpose.

摘要

骨再生是解决大组织缺损性骨缺损的一项挑战性课题，这涉及骨移植来支持该活动。已对细菌纤维素改性的聚羟基链烷酸酯（PHB/BC）支架的体外生物相容性及其在临界尺寸小鼠颅骨缺损中的成骨潜力进行了研究。通过生物化学、组织学/组织形态计量学、X射线分析和免疫荧光分析骨促进和矿化情况，以突出成骨标志物。总之，我们的结果表明，PHB/BC支架能够支持3T3-L1前脂肪细胞增殖，并对体内成骨细胞分化有积极作用，从而在植入后20周诱导新骨形成。因此，新开发的PHB/BC支架可能成为适用于骨组织工程目的的生物材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/eb5fc2333ea4/materials-13-01433-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/9d2c6dec9e2a/materials-13-01433-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/0e2db3ea63e7/materials-13-01433-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/2eba787021dd/materials-13-01433-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/2211d1f27804/materials-13-01433-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/5c036ed530b8/materials-13-01433-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/de500b403a3b/materials-13-01433-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/eb5fc2333ea4/materials-13-01433-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/9d2c6dec9e2a/materials-13-01433-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/41fd8e95c94f/materials-13-01433-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/223fed948c65/materials-13-01433-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/0e2db3ea63e7/materials-13-01433-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/2eba787021dd/materials-13-01433-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/2211d1f27804/materials-13-01433-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/5c036ed530b8/materials-13-01433-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/de500b403a3b/materials-13-01433-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8951/7142421/eb5fc2333ea4/materials-13-01433-g009.jpg

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