Lo S J, Chen M L, Chien M L, Lee Y H
Biochem Biophys Res Commun. 1986 Mar 13;135(2):382-8. doi: 10.1016/0006-291x(86)90006-9.
The nucleotide sequence of our cloned HBV DNA (subtype adw) has been determined. When the 165-nucleotide sequence of the pre-S2 region was compared with 7 other published sequences (subtypes adw, adr, ayw, and adyw), we found 38 nucleotide substitutions among different subtypes and 4 (adr) or 6 (adw and ayw) substitutions within the same subtype. Analysis of the predicted amino acid sequence from the known nucleotide sequences indicates that: there are 20 amino acid substitutions, the longest conserved amino acid sequence is located between amino acids 23 to 34, and the 54th amino acid is identical within the same subtype but varies in different subtypes.
我们克隆的乙肝病毒（HBV）DNA（adw亚型）的核苷酸序列已被确定。当将前S2区的165个核苷酸序列与其他7个已发表的序列（adw、adr、ayw和adyw亚型）进行比较时，我们发现在不同亚型之间有38个核苷酸替换，在同一亚型内有4个（adr）或6个（adw和ayw）替换。对已知核苷酸序列预测的氨基酸序列分析表明：有20个氨基酸替换，最长的保守氨基酸序列位于第23至34个氨基酸之间，第54个氨基酸在同一亚型内相同，但在不同亚型中有所不同。
