乙型肝炎病毒DNA adr亚型的克隆与结构分析
Cloning and structural analyses of hepatitis B virus DNAs, subtype adr.
作者信息
Fujiyama A, Miyanohara A, Nozaki C, Yoneyama T, Ohtomo N, Matsubara K
出版信息
Nucleic Acids Res. 1983 Jul 11;11(13):4601-10. doi: 10.1093/nar/11.13.4601.
Abstract
Entire genomes of hepatitis B virus (subtype adr) have been cloned. The nucleotide sequence data were compared with other sequences of HBV genome including: adw [Valenzuela et al. (1981) in Animal Virus Genetics. Fields et al. eds. Academic Press, Inc., NY. pp. 57-70], ayw [Galibert et al. (1979) Nature, 281, 646-650], and adyw [Pasek et al. (1979) Nature 282, 575-579]. Four open coding frames for polypeptides larger than 6,000 dalton were found to be conserved and were highly compressed by overlapping with each other in one strand (L-strand). Sites of initiation of the S gene and termination of the P gene were not conserved. No conserved coding frame was found on the opposite strand (S strand). Amino acid sequences of six surface antigen (HBsAg) peptides, including subtypes adr, adw, and ayw, are deduced from the DNA sequences, and the substitution of amino acid residues which are consistent with the change of subtypes are demonstrated.
摘要
乙型肝炎病毒（adr亚型）的全基因组已被克隆。将核苷酸序列数据与乙肝病毒基因组的其他序列进行了比较，这些序列包括：adw [巴伦苏埃拉等人（1981年），载于《动物病毒遗传学》。菲尔兹等人编著。学术出版社，纽约。第57 - 70页]，ayw [加利贝尔等人（1979年），《自然》，281卷，646 - 650页]，以及adyw [帕塞克等人（1979年），《自然》282卷，575 - 579页]。发现了四个编码大于6000道尔顿多肽的开放阅读框是保守的，并且在一条链（L链）上彼此重叠从而高度压缩。S基因的起始位点和P基因的终止位点并不保守。在相反的链（S链）上未发现保守的编码框。从DNA序列推导了六种表面抗原（HBsAg）肽的氨基酸序列，包括adr、adw和ayw亚型，并证明了与亚型变化一致的氨基酸残基替换。
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