Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Veterinary Medical Center, The University of Tokyo, Tokyo, Japan.
J Vet Med Sci. 2023 Oct 18;85(10):1068-1073. doi: 10.1292/jvms.23-0062. Epub 2023 Aug 4.
Podoplanin (PDPN) is a prognostic factor and is involved in several mechanisms of tumor progression in human squamous cell carcinoma (SCC). Canine non-tonsillar SCC (NTSCC) is a common oral tumor in dogs and has a highly invasive characteristic. In this study, we investigated the function of PDPN in canine NTSCC. In canine NTSCC clinical samples, PDPN overexpression was observed in 80% of dogs with NTSCC, and PDPN expression was related to ki67 expression. In PDPN knocked-out canine NTSCC cells, cell proliferation, cancer stemness, and migration were suppressed. As the mechanism of PDPN-mediated cell proliferation, PDPN knocked-out induced apoptosis and G2/M cell cycle arrest in canine NTSCC cells. These findings suggest that PDPN promotes tumor malignancies and may be a novel biomarker and therapeutic target for canine NTSCC.
足突蛋白 (PDPN) 是一种预后因素，参与了人类鳞状细胞癌 (SCC) 中的几种肿瘤进展机制。犬非扁桃体 SCC (NTSCC) 是犬中常见的口腔肿瘤，具有高度侵袭性的特征。在这项研究中，我们研究了 PDPN 在犬 NTSCC 中的功能。在犬 NTSCC 的临床样本中，80%的 NTSCC 犬存在 PDPN 过表达，并且 PDPN 的表达与 ki67 的表达相关。在 PDPN 敲除的犬 NTSCC 细胞中，细胞增殖、癌症干性和迁移受到抑制。作为 PDPN 介导的细胞增殖的机制，PDPN 敲除诱导了犬 NTSCC 细胞的细胞凋亡和 G2/M 细胞周期阻滞。这些发现表明 PDPN 促进了肿瘤恶性进展，可能成为犬 NTSCC 的一种新的生物标志物和治疗靶标。
