Marani E
Stain Technol. 1978 Sep;53(5):265-8. doi: 10.3109/10520297809111943.
Tissue for cryostat sectioning is embedded together with reference tissue impaled on the needles of a specially constructed trident. To maintain spatial orientation, reference and experimental tissue are frozen in Tissue-Tek O. C. T. compound in the manner usual for enzyme histochemistry using a simply constructed carrier. The trident is removed by heating its handle, thus leaving holes in the reference tissue which in turn are used as reference points to orient successive sections with respect to each other.
用于低温恒温器切片的组织与固定在特制三叉戟针上的参照组织一起包埋。为保持空间定向，参照组织和实验组织以酶组织化学常用的方式，在Tissue-Tek O.C.T. 化合物中，使用简易的载体进行冷冻。通过加热三叉戟的手柄将其移除，从而在参照组织上留下孔洞，这些孔洞进而用作参照点，使连续的切片能够相互定向。
