子宫内接受X射线照射的胎鼠大脑中的超氧化物歧化酶

Superoxide dismutase in fetal rat cerebrum X-irradiated in utero.

作者信息

Suzuki H, Tanaka H, Iwasaki S, Arima M

出版信息

Brain Dev. 1986;8(1):17-24. doi: 10.1016/s0387-7604(86)80115-2.

Abstract

The superoxide dismutase (SOD) activity in fetal rat cerebrum was studied on gestational days (gd) 19 and 21 after X-irradiation with 100R on gd 13 or no X-irradiation. Fetuses X-irradiated on gd 13 showed marked microcephalus on gd 19 and 21. Most of the SOD activity was found in the crude mitochondrial fraction in both groups and most of the SOD was Cu,Zn-SOD. The SOD activity increased about two times from gd 19 to 21. The mean SOD activity of the fetal rat cerebrum X-irradiated with 100R showed no significant differences compared to that of the control group with Student's t test. But the activities in the irradiated group were widely distributed with a large standard deviation and so the chi-square test showed a significant difference in SOD activity between the two groups on gd 19. The SOD activity of adult rat cerebrum 6 and 8 days after X-irradiation with 100R was increased compared to that 3 days after X-irradiation. It was suspected that X-irradiation in utero may have some lasting effect on the SOD activity in fetal rat cerebrum.

摘要

在妊娠第13天对胎鼠进行100伦琴的X射线照射或不进行X射线照射后，研究了妊娠第19天和第21天胎鼠大脑中超氧化物歧化酶（SOD）的活性。在妊娠第13天接受X射线照射的胎儿在第19天和第21天出现明显的小头畸形。两组中大部分SOD活性存在于粗线粒体组分中，且大部分SOD为铜锌超氧化物歧化酶。从第19天到第21天，SOD活性增加了约两倍。用学生t检验，接受100伦琴X射线照射的胎鼠大脑的平均SOD活性与对照组相比无显著差异。但照射组的活性分布广泛，标准差较大，因此卡方检验显示两组在第19天的SOD活性存在显著差异。与X射线照射后3天相比，成年大鼠大脑在接受100伦琴X射线照射后6天和8天的SOD活性增加。怀疑子宫内的X射线照射可能对胎鼠大脑中的SOD活性有一些持久影响。