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用于热带奶牛基因组选择的胚胎活检

Embryo biopsies for genomic selection in tropical dairy cattle.

作者信息

Oliveira Clara Slade, Camargo Luiz Sergio Almeida, da Silva Marcos Vinicius Gualberto Barbosa, Saraiva Naiara Zoccal, Quintão Carolina Capobiango, Machado Marco Antonio

机构信息

Embrapa Gado de Leite, Juiz de Fora, MG, Brasil.

出版信息

Anim Reprod. 2023 Jul 24;20(2):e20230064. doi: 10.1590/1984-3143-AR2023-0064. eCollection 2023.

Abstract

Genomic selection has transformed the livestock industry, enabling early-life selection of animals. Biopsy sampling of pre-implantation embryos has been described since 1968. However, it was only after 2010, with the advancement of molecular biology techniques such as whole genomic amplification and SNP Chips, that next-generation sequencing became commercially available for bovine embryos. It is now possible to make decisions about which embryos to transfer not only based on recipients' availability or embryo morphology but also on genomic estimates. This technology can be implemented for a wide spectrum of applications in livestock. In this review, we discuss the use of embryo biopsy for genomic selection and share our experience with Gir and Girolando Brazilian breeding programs, as well as future goals for implementing it in Brazilian bovine embryo production practices.

摘要

基因组选择已经改变了畜牧业,实现了对动物的早期选择。自1968年以来,就有关于植入前胚胎活检取样的描述。然而,直到2010年以后,随着全基因组扩增和单核苷酸多态性芯片等分子生物学技术的进步,下一代测序才开始在商业上应用于牛胚胎。现在,不仅可以根据受体的可用性或胚胎形态,还可以根据基因组估计来决定移植哪些胚胎。这项技术可用于畜牧业的广泛应用。在这篇综述中,我们讨论了胚胎活检在基因组选择中的应用,并分享了我们在巴西吉尔牛和吉罗兰多牛育种计划中的经验,以及在巴西牛胚胎生产实践中实施该技术的未来目标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f43f/10399131/28894cc8eb0f/1984-3143-ar-20-2-e20230064-gf01.jpg

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