Department of Pediatrics, Peking University First Hospital, Beijing, China.
State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences, Beijing, Beijing Institute of Lifeomics, Beijing, China.
Antioxid Redox Signal. 2024 Jan;40(1-3):145-167. doi: 10.1089/ars.2023.0407. Epub 2023 Sep 5.
Gasotransmitters, including nitric oxide (NO), hydrogen sulfide (HS) and sulfur dioxide (SO), participate in various cellular processes corresponding oxidative posttranslational modifications (oxiPTMs) of specific cysteines. Accumulating evidence has clarified the mechanisms underlying the formation of oxiPTMs derived from gasotransmitters and their biological functions in multiple signal pathways. Because of the specific existence and functional importance, determining the sites of oxiPTMs in cysteine is crucial in biology. Recent advances in the development of selective probes, together with upgraded mass spectrometry (MS)-based proteomics, have enabled the quantitative analysis of cysteinome. To date, several cysteine residues have been identified as gasotransmitter targets. To clearly understand the underlying mechanisms for gasotransmitter-mediated biological processes, it is important to identify modified targets. In this review, we summarize the chemical formation and biological effects of gasotransmitter-dependent oxiPTMs and highlight the state-of-the-art detection methods. Future studies in this field should aim to develop the next generation of probes for labeling to improve spatial resolution and determine the dynamic change of oxiPTMs, which can lay the foundation for research on the molecular mechanisms and clinical translation of gasotransmitters. 40, 145-167.
气体递质，包括一氧化氮（NO）、硫化氢（HS）和二氧化硫（SO），参与各种细胞过程，对应于特定半胱氨酸的氧化后翻译修饰（oxiPTMs）。越来越多的证据阐明了源自气体递质的 oxiPTMs 的形成机制及其在多种信号通路中的生物学功能。由于特定的存在和功能重要性，确定半胱氨酸中 oxiPTMs 的位点在生物学中至关重要。选择性探针的开发的最新进展，以及升级的基于质谱（MS）的蛋白质组学，使半胱氨酸组的定量分析成为可能。迄今为止，已经鉴定出几个半胱氨酸残基作为气体递质的靶标。为了清楚地理解气体递质介导的生物学过程的潜在机制，识别修饰的靶标非常重要。在这篇综述中，我们总结了气体递质依赖性 oxiPTMs 的化学形成和生物学效应，并强调了最新的检测方法。该领域的未来研究应旨在开发下一代用于标记的探针，以提高空间分辨率并确定 oxiPTMs 的动态变化，这可为气体递质的分子机制研究和临床转化奠定基础。
