基于氢核磁共振代谢组学的中国儿童营养不良严重程度相关潜在生物标志物的鉴定：一项初步研究。
Identification of potential biomarkers in malnutrition children with severity by H-NMR-based metabolomics: a preliminary study in the Chinese population.
机构信息
Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, 422 Siming South Road, Xiamen, 361005, Fujian, China.
Department of Child Health, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, 361003, Fujian, China.
出版信息
Eur J Nutr. 2023 Dec;62(8):3193-3205. doi: 10.1007/s00394-023-03224-7. Epub 2023 Aug 7.
PURPOSE
Child malnutrition is a global public health problem, but the underlying pathophysiologic mechanisms with severity remain poorly understood, and the potential biomarkers served to the clinical diagnosis are still not available. This study aimed to identify the serum metabolic characteristics of malnourished children with severity.
METHODS
Fasted overnight serum samples were collected following clinical standard procedures among 275 malnourished and 199 healthy children from the Women and Children's Hospital, Xiamen University Child Health Department from July 2020 to May 2022. Nuclear magnetic resonance (NMR)-based metabolomics strategy was applied to identify the potential serum biomarkers of malnutrition from 275 malnourished children aged 4 to 84 months with mild (Mil, 199 cases), moderate (Mod, 101 cases), and severe (Sev, 7 cases) malnutrition.
RESULTS
Ten, fifteen, and fifteen differential metabolites were identified from the Mil, Mod, and Sev malnutrition groups, respectively. Eight common metabolites, including increased acetoacetate, acetone, ethanol, succinate, 3-hydroxybutyrate, and decreased alanine, methionine, and N-acetyl-glycoprotein, could be the potential biomarkers for malnourished children. The altered metabolic pathways were mainly related to energy metabolism and amino acid metabolism via the network-based pathway enrichment.
CONCLUSION
Eight potential biomarkers, including acetoacetate, acetone, ethanol, succinate, 3-hydroxybutyrate, alanine, methionine, and N-acetyl-glycoprotein, could characterize the child malnutrition. Child malnutrition-induced abnormal energy metabolism, impaired nutrition utilization and the reduced nutrient availability, and more metabolic disturbance will appear with the severity. Our results are valuable for further studies on the etiology and pathogenesis of malnutrition for clinical intervention and improvement.
目的
儿童营养不良是一个全球性的公共卫生问题，但严重程度的潜在病理生理机制仍了解甚少，潜在的生物标志物也尚未用于临床诊断。本研究旨在确定严重营养不良儿童的血清代谢特征。
方法
2020 年 7 月至 2022 年 5 月，从厦门大学附属妇女儿童医院儿童保健科收集了 275 名营养不良和 199 名健康儿童的空腹过夜血清样本。采用基于核磁共振（NMR）的代谢组学策略，从 275 名 4 至 84 个月大的轻度（Mil，199 例）、中度（Mod，101 例）和重度（Sev，7 例）营养不良儿童中鉴定出潜在的营养不良血清生物标志物。
结果
从 Mil、Mod 和 Sev 营养不良组分别鉴定出 10、15 和 15 个差异代谢物。8 个共同的代谢物，包括升高的乙酰乙酸、丙酮、乙醇、琥珀酸、3-羟基丁酸和降低的丙氨酸、蛋氨酸和 N-乙酰糖蛋白，可能是营养不良儿童的潜在生物标志物。改变的代谢途径主要与能量代谢和氨基酸代谢有关，通过网络通路富集分析。
结论
8 个潜在的生物标志物，包括乙酰乙酸、丙酮、乙醇、琥珀酸、3-羟基丁酸、丙氨酸、蛋氨酸和 N-乙酰糖蛋白，可用于描述儿童营养不良。儿童营养不良引起的异常能量代谢、营养利用受损和营养物质供应减少，以及更多的代谢紊乱，随着严重程度的增加而出现。我们的研究结果对于进一步研究营养不良的病因和发病机制，以及为临床干预和改善提供了有价值的参考。
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