Loganathan Archana, Nachimuthu Ramesh
Department of Bio-Medical Sciences, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu 632014, India.
Asian Biomed (Res Rev News). 2022 Aug 31;16(4):191-199. doi: 10.2478/abm-2022-0023. eCollection 2022 Aug.
is a pathogen endemic in India and sometimes deadly for patients in intensive care units.
To determine the antibiotic-resistance pattern, biofilm forming ability, and clonal type of from isolates collected in Tamil Nadu (south) and the Mizoram (northeast) regions of India.
We collected isolates from diagnostic laboratories in Tamil Nadu and Mizoram. An antibiotic susceptibility test was performed according to Clinical Laboratory and Standards Institute methods. Antibiotic-resistant determinants such as , , , , , and were confirmed by polymerase chain reaction (PCR). All isolates were further studied for biofilm forming ability. Enterobacterial repetitive intergenic consensus (ERIC)-PCR was used for clonal analysis.
A study of 206 clinical isolates showed 52.9% prevalence of methicillin-resistant in Tamil Nadu and 49.4% in Mizoram. Minimum inhibitory concentration tests showed a high prevalence of 67% oxacillin resistance in isolates from Tamil Nadu and 49% in isolates from Mizoram. PCR showed 53% in Tamil Nadu and 49% in Mizoram. Vancomycin-intermediate resistance (VISA) prevalence was lower in isolates from Tamil Nadu (4%) and Mizoram (5%). All methicillin-resistant (MRSA) isolates formed biofilms. Clonal analysis revealed a genetic relatedness between the isolates.
The prevalence of MRSA is high in the regions studied, with most of the clinical isolates being multidrug resistant. Adopting appropriate community-based preventive measures and establishing antimicrobial stewardship is highly recommended to minimize the dissemination in antibiotic resistance.
是一种在印度流行的病原体，有时对重症监护病房的患者具有致命性。
确定从印度泰米尔纳德邦（南部）和米佐拉姆邦（东北部）收集的分离株的抗生素耐药模式、生物膜形成能力和克隆类型。
我们从泰米尔纳德邦和米佐拉姆邦的诊断实验室收集分离株。根据临床实验室和标准协会的方法进行抗生素敏感性试验。通过聚合酶链反应（PCR）确认抗生素耐药决定因素，如、、、、和。对所有分离株进一步研究其生物膜形成能力。使用肠杆菌重复基因间共识（ERIC）-PCR进行克隆分析。
对206株临床分离株的研究表明，泰米尔纳德邦耐甲氧西林的患病率为52.9%，米佐拉姆邦为49.4%。最低抑菌浓度试验显示，泰米尔纳德邦分离株中67%对苯唑西林耐药，米佐拉姆邦分离株中49%耐药。PCR显示，泰米尔纳德邦为53%，米佐拉姆邦为49%。泰米尔纳德邦（4%）和米佐拉姆邦（5%）分离株中万古霉素中介耐药（VISA）的患病率较低。所有耐甲氧西林（MRSA）分离株均形成生物膜。克隆分析揭示了分离株之间的遗传相关性。
在所研究的地区，MRSA的患病率很高，大多数临床分离株具有多重耐药性。强烈建议采取适当的基于社区的预防措施并建立抗菌药物管理，以尽量减少抗生素耐药性的传播。
