Ozmen Ozlem, Tomul Fatma, Sirin Yusuf Sinan
Department of Pathology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Istiklal Yerleskesi, 15030 Burdur, Turkey.
Department of Chemistry, Faculty of Art and Science, Burdur Mehmet Akif Ersoy University, Istiklal Yerleskesi, 15030 Burdur, Turkey.
Asian Biomed (Res Rev News). 2023 Jun 16;16(5):237-248. doi: 10.2478/abm-2022-0027. eCollection 2022 Oct.
Enhancing the bone healing procedure would resultantly improve the post-recovery life quality, as well as the speed with which the patient returns to their former life quality. Porous structures can provide a large surface area and abundant channels to facilitate mass transfer.
To evaluate the application of mesoporous materials in the bone healing of surgically created defects on the tibiae of male adult Wistar rats.
The defect areas were evaluated after implantation of 4 types of bioactive glass histopathologically and immunohistochemically. Fifty adult rats were divided into 5 groups including a control group without material. The used products were mesoporous bioactive glass (MBG), Cu-MBG, Zn-MBG, and Cu-Zn-MBG. Unicortical bone defects with a 3 mm diameter were performed in both tibiae of the animals and filled with 4 types of glass particles. The rats were then euthanized at 15 d and 30 d. Tibial samples were collected and the tissues forwarded for histological processing, and examined using light microscopy. Additionally, bone healing was evaluated by assessing the levels of bone morphogenetic protein BMP2, collagen 1, osteocalcin (OST), and vascular endothelial growth factor (VEGF) using immunohistochemical methods.
Within the 15th day, all groups presented connective tissue septa; at the 30th day, the new bone formation was more intense in the Cu-Zn-MBG group. Additionally, BMP2, collagen 1, OST, and VEGF immune expression were more prominent in the Cu-Zn-MBG group.
The study results indicated that MBG may be used for the repairing of bone defects. Cu-Zn-MBG may be the best choice for this purpose.
促进骨愈合过程将相应地提高恢复后的生活质量，以及患者恢复到以前生活质量的速度。多孔结构可以提供大的表面积和丰富的通道以促进物质传递。
评估介孔材料在雄性成年Wistar大鼠胫骨手术造成的缺损骨愈合中的应用。
植入4种生物活性玻璃后，通过组织病理学和免疫组织化学方法评估缺损区域。50只成年大鼠分为5组，包括一个不植入材料的对照组。所用产品为介孔生物活性玻璃（MBG）、铜掺杂介孔生物活性玻璃（Cu-MBG）、锌掺杂介孔生物活性玻璃（Zn-MBG）和铜锌掺杂介孔生物活性玻璃（Cu-Zn-MBG）。在动物的双侧胫骨上制造直径为3 mm的单皮质骨缺损，并用4种玻璃颗粒填充。然后在第15天和第30天对大鼠实施安乐死。收集胫骨样本，将组织送去进行组织学处理，并使用光学显微镜进行检查。此外，通过免疫组织化学方法评估骨形态发生蛋白BMP2、胶原蛋白1、骨钙素（OST）和血管内皮生长因子（VEGF）的水平来评估骨愈合情况。
在第15天时，所有组均出现结缔组织间隔；在第30天时，Cu-Zn-MBG组的新骨形成更为明显。此外，Cu-Zn-MBG组中BMP2、胶原蛋白1、OST和VEGF的免疫表达更为突出。
研究结果表明，MBG可用于修复骨缺损。Cu-Zn-MBG可能是用于此目的的最佳选择。
