Key Laboratory of Oral Diseases Research of Anhui Province, College ＆ Hospital of Stomatology, Anhui Medical University, Hefei 230032, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2021 Dec 1;39(6):667-674. doi: 10.7518/hxkq.2021.06.007.
This study was performed to evaluate the occlusion of monetite paste on dentine tubule and provide a new potential method for treating dentine hypersensitivity.
Calcium oxide, strontium chloride, and polyethylene glycol phosphate were mixed in a certain proportion and ground in a planetary ball mill. The reaction was carried out by adjusting the pH to obtain monetite and hydroxyapatite paste. The morphological characteristics of the paste were observed through scanning electron microscope (SEM). The structure and composition were analyzed through X-ray diffraction (XRD) and Fourier transform infrared spectrometer (FTIR). The extracted third molar was selected to undergo demineralization to establish the study model of dentin hypersensitivity. The samples were randomly divided into four groups: blank control group (treated with distilled water), casein peptide phosphate-amorphic calcium phosphate (CPP-ACP) group, monetite paste group, and hydroxyapatite paste group. Each group was used to scrub the dentin surface with the corresponding materials for 7 days. The morphological characteristics of the dentin surface and section were observed through SEM, the microhardness of the dentin before and after mineralization was analyzed with a microhardness tester, and the composition of the deposits on the surface of the mineralized samples was examined through XRD.
XRD and FTIR showed that the composition of the paste was mainly monetite, and the composition of hydroxyapatite paste was mainly composed of hydroxyapatite. SEM revealed that the size of the crystal particles of the synthesized paste was tens to hundreds of nanometers. Monetite and hydroxyapatite paste could produce a thicker mineralization layer on the dentin surface, and the mineralization of the dentin tubules of monetite was deeper than that of hydroxyapatite paste. The microhardness of the monetite paste group was significantly less than those of the hydroxyapatite paste groups (<0.05).
Monetite paste could effectively block the exposed dentin tubules and be used for treating dentin hypersensitivity.
本研究旨在评估磷灰石糊剂对牙本质小管的阻塞作用，并为治疗牙本质敏感症提供一种新的潜在方法。
将氧化钙、氯化锶和聚乙二醇磷酸盐按一定比例混合，在行星球磨机中研磨。通过调整 pH 值进行反应，得到磷灰石和羟磷灰石糊剂。通过扫描电子显微镜（SEM）观察糊剂的形态特征。通过 X 射线衍射（XRD）和傅里叶变换红外光谱（FTIR）分析其结构和组成。选择提取的第三磨牙进行脱矿处理，建立牙本质敏感症的研究模型。将样本随机分为 4 组：空白对照组（用蒸馏水处理）、酪蛋白磷酸肽-无定形磷酸钙（CPP-ACP）组、磷灰石糊剂组和羟磷灰石糊剂组。每组用相应材料擦洗牙本质表面 7 天。通过 SEM 观察牙本质表面和剖面的形态特征，用显微硬度计分析矿化前后牙本质的显微硬度，通过 XRD 检查矿化样品表面沉积物的组成。
XRD 和 FTIR 表明糊剂的主要成分为磷灰石，羟磷灰石糊剂的主要成分为羟磷灰石。SEM 显示合成糊剂的晶体颗粒尺寸为数十到数百纳米。磷灰石和羟磷灰石糊剂均可在牙本质表面产生较厚的矿化层，且磷灰石对牙本质小管的矿化深度大于羟磷灰石糊剂。磷灰石糊剂组的显微硬度明显小于羟磷灰石糊剂组（<0.05）。
磷灰石糊剂能有效封闭暴露的牙本质小管，可用于治疗牙本质敏感症。
