Burchard G D, Albiez E J, Bierther M
Tropenmed Parasitol. 1979 Mar;30(1):97-102.
Skin snips of six patients with manifest onchocerciasis treated with a single dose of metrifonate (10 mg/kg) were examined by electron microscopy one, three, five and ten days after application of the drug. Compared to untreated cases 24 hours after treatment there is a marked increase in the extend of perivascular infiltrates which are composed not only of macrophages, lymphocytes and plasma cells, but also of eosinophils. Infiltrates directly around dead microfilariae are seen infrequently. This pattern of cellular reaction remains constant for the first ten days after treatment. Metrifonate obviously kills the microfilariae rapidly, already 24 hours after its application totally disintegrated parasites are to be found. On the other hand always a few intact microfilariae are seen, possibly they just hatched after treatment.
对6例明显盘尾丝虫病患者单次服用敌百虫（10毫克/千克）进行治疗，并于用药后1天、3天、5天和10天通过电子显微镜检查皮肤切片。与治疗24小时后未治疗的病例相比，血管周围浸润范围明显增加，这些浸润不仅由巨噬细胞、淋巴细胞和浆细胞组成，还包括嗜酸性粒细胞。很少见到直接围绕死亡微丝蚴的浸润。这种细胞反应模式在治疗后的前10天保持不变。敌百虫显然能迅速杀死微丝蚴，用药后24小时即可发现完全解体的寄生虫。另一方面，总是能看到一些完整的微丝蚴，可能它们是在治疗后刚刚孵化出来的。
