State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China.
State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China.
Cell Rep. 2023 Aug 29;42(8):112938. doi: 10.1016/j.celrep.2023.112938. Epub 2023 Aug 6.
Increasing plant resistance to Verticillium wilt (VW), which causes massive losses of Brassica rapa crops, is a challenge worldwide. However, few causal genes for VW resistance have been identified by forward genetic approaches, resulting in limited application in breeding. We combine a genome-wide association study in a natural population and quantitative trait locus mapping in an F2 population and identify that the MYB transcription factor BrMYB108 regulates plant resistance to VW. A 179 bp insertion in the BrMYB108 promoter alters its expression pattern during Verticillium longisporum (VL) infection. High BrMYB108 expression leads to high VL resistance, which is confirmed by disease resistance tests using BrMYB108 overexpression and loss-of-function mutants. Furthermore, we verify that BrMYB108 confers VL resistance by regulating reactive oxygen species (ROS) generation through binding to the promoters of respiratory burst oxidase genes (Rboh). A loss-of-function mutant of AtRbohF in Arabidopsis shows significant susceptibility to VL. Thus, BrMYB108 and its target ROS genes could be used as targets for genetic engineering for VL resistance of B. rapa.
提高对黄萎病（VW）的植物抗性，这会导致油菜作物的大量损失，这是全世界的一个挑战。然而，通过正向遗传学方法已经确定了少数与 VW 抗性相关的因果基因，这导致其在育种中的应用有限。我们结合了自然群体中的全基因组关联研究和 F2 群体中的数量性状位点作图，鉴定出 MYB 转录因子 BrMYB108 调节植物对 VW 的抗性。BrMYB108 启动子中的 179bp 插入改变了其在黄萎轮枝菌（VL）感染过程中的表达模式。高 BrMYB108 表达导致对 VL 的高抗性，这通过使用 BrMYB108 过表达和功能丧失突变体进行抗病性测试得到了证实。此外，我们通过验证 BrMYB108 通过与呼吸爆发氧化酶基因（Rboh）的启动子结合来调节活性氧（ROS）的产生来赋予 VL 抗性。拟南芥中 AtRbohF 的功能丧失突变体对 VL 表现出明显的敏感性。因此，BrMYB108 及其靶标 ROS 基因可以作为油菜 VW 抗性遗传工程的靶点。
