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胰蛋白胨-酵母提取物肉汤作为胸膜肺炎放线杆菌和副猪嗜血杆菌的培养基,用作攻毒接种物。

Tryptone-yeast extract broth as a culture medium for Haemophilus pleuropneumoniae and Haemophilus parasuis to be used as challenge inocula.

作者信息

O'Reilly T, Niven D F

出版信息

Can J Vet Res. 1986 Jul;50(3):441-3.

PMID:3755642
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1255242/
Abstract

Haemophilus pleuropneumoniae and Haemophilus parasuis were grown in a liquid medium containing tryptone and yeast extract. Whereas culture turbidity during exponential growth of either organism was proportional to the number of viable cells, continued incubation of stationary phase cultures was accompanied by a dramatic decrease in viability with no corresponding decrease in culture turbidity. It is concluded that the medium described should prove useful for the growth of porcine haemophili for use in experimental infections provided the stage of growth is given due consideration.

摘要

胸膜肺炎放线杆菌和副猪嗜血杆菌在含有胰蛋白胨和酵母提取物的液体培养基中培养。虽然任何一种细菌指数生长期间的培养物浊度与活细胞数量成正比,但对稳定期培养物继续培养时,存活率会急剧下降,而培养物浊度却没有相应降低。得出的结论是,只要适当考虑生长阶段,所述培养基应被证明对用于实验性感染的猪嗜血杆菌的生长有用。

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本文引用的文献

1
Effect of temperature on the survival of Haemophilus parasuis in physiological saline.温度对副猪嗜血杆菌在生理盐水中存活的影响。
Natl Inst Anim Health Q (Tokyo). 1982 Summer;22(2):90-1.
2
Antimicrobial susceptibility of 51 strains of Haemophilus pleuropneumoniae.51株胸膜肺炎放线杆菌的药敏试验
Can J Comp Med. 1984 Jan;48(1):47-50.
3
Porcine haemophili and actinobacilli: characterization by means of API test strips and possible taxonomic implications.
Can J Microbiol. 1984 Oct;30(10):1229-38. doi: 10.1139/m84-195.
4
The cytochrome complement of Haemophilus parasuis.
Can J Microbiol. 1984 Jun;30(6):763-73. doi: 10.1139/m84-117.
5
Haemophilus pleuropneumoniae infection in swine: a review.猪胸膜肺炎放线杆菌感染:综述
J Am Vet Med Assoc. 1983 Jun 15;182(12):1331-7.
6
Evaluation of a selective medium for isolation of Haemophilus pleuropneumoniae.用于分离猪胸膜肺炎放线杆菌的选择性培养基的评估
Can J Comp Med. 1983 Oct;47(4):445-50.
7
The generalized Shwartzman reaction and Haemophilus infections in pigs.猪的全身性施瓦茨曼反应与嗜血杆菌感染
Pathol Vet. 1967;4(3):245-53. doi: 10.1177/030098586700400304.
8
Defining the metabolic and growth responses of porcine haemophili to exogenous pyridine nucleotides and precursors.确定猪血友病对外源吡啶核苷酸及其前体的代谢和生长反应。
J Gen Microbiol. 1986 Mar;132(3):807-18. doi: 10.1099/00221287-132-3-807.
9
Production of RNA-dependent haemolysin by Haemophilus pleuropneumoniae.胸膜肺炎放线杆菌产生RNA依赖性溶血素。
Can J Microbiol. 1985 May;31(5):456-62. doi: 10.1139/m85-085.