Martin P G, Lachance P, Niven D F
Can J Microbiol. 1985 May;31(5):456-62. doi: 10.1139/m85-085.
Five strains of Haemophilus pleuropneumoniae, out of eight strains tested, produced extracellular haemolysin(s) when grown in liquid culture in the presence, but not in the absence, of RNA. The haemolysin produced by the neotype strain was unstable, heat labile, and sensitive to degradation by pronase, trypsin, and chymotrypsin; moreover, trypan blue treated haemolysin preparations were less effective at causing erythrocyte lysis than were untreated preparations. Following growth in the absence of RNA, washed suspensions of the neotype strain produced extracellular haemolysin when incubated in the presence of RNA, glucose, and casein acid hydrolysate; extracellular haemolysin could not be detected if the incubation mixture contained chloramphenicol. The haemolysin produced by washed bacterial suspensions was similar to that produced by growing cultures in that it was unstable, heat labile, and sensitive to inactivation by the same complement of enzymes. Erythrocyte lysis induced by either haemolysin preparation was preceded by a prelytic phase, the duration of which was dependent upon haemolysin concentration and the initial temperature of the haemolysin--erythrocyte mixture. It is concluded that the haemolysin(s) produced by the neotype strain of H. pleuropneumoniae is distinct from, but closely related to both streptolysin S and the haemolysin produced by Treponema hyodysenteriae.
在检测的8株猪胸膜肺炎放线杆菌中，有5株在含RNA的液体培养基中生长时可产生细胞外溶血素，而在不含RNA的培养基中则不产生。新血清型菌株产生的溶血素不稳定、对热敏感，且对链霉蛋白酶、胰蛋白酶和糜蛋白酶的降解敏感；此外，经台盼蓝处理的溶血素制剂引起红细胞溶解的效果不如未处理的制剂。在不含RNA的培养基中生长后，新血清型菌株的洗涤悬浮液在RNA、葡萄糖和酪蛋白酸水解物存在的情况下孵育时会产生细胞外溶血素；如果孵育混合物中含有氯霉素，则无法检测到细胞外溶血素。洗涤后的细菌悬浮液产生的溶血素与生长培养物产生的溶血素相似，即不稳定、对热敏感，且对相同的一组酶的失活敏感。两种溶血素制剂诱导的红细胞溶解之前都有一个溶血前期，其持续时间取决于溶血素浓度和溶血素-红细胞混合物的初始温度。结论是，猪胸膜肺炎放线杆菌新血清型菌株产生的溶血素与链球菌溶血素S以及猪痢疾密螺旋体产生的溶血素不同，但密切相关。
