School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China.
Institute of Functional Nano and Soft Materials Laboratory (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, Jiangsu, China.
Colloids Surf B Biointerfaces. 2023 Oct;230:113492. doi: 10.1016/j.colsurfb.2023.113492. Epub 2023 Aug 4.
Alcoholic gastric ulcer is a common acute gastric injury disease. The drugs currently used in clinical practice not only cannot fundamentally treat gastric injury, but also have serious side effects. There is an urgent demand for the discovery of a mild drug to treat alcoholic gastric ulcers. Herein, the green carbon dots derived from charred Atractylodes macrocephala (CAM-CDs) were acquired and have been proven to be safe and effective in alleviating alcoholic gastric ulcers at an inhibition rate up to 60%. CAM-CDs can markedly attenuate the gastric mucosa damage such as mucosal defect, bleeding and inflammatory cell infiltration by histopathological examination. Serum and tissue inflammatory cytokine measurements, as well as immunohistochemistry results, indicate that its mechanism of gastric mucosal protection may involve the reduction of IL-1β and TNF-α by regulating inflammatory signaling pathway of the NF-κB/NLRP3 axis, as well as elevation of IL-10 levels. CAM-CDs also can reduce oxidative stress markers (MDA), increase PGE2 and mucin secretion (MUC5AC), and it simultaneously exerts slight inhibition of hydrogen potassium ATPase and pepsin activity to protect gastric mucosa, as well as increases the microbial diversity and regulates species composition of gut microbiota in rats with gastric ulcer. Our work provides a new perspective on utilizing carbon-based nanomaterials in the development of new mild drugs.
酒精性胃溃疡是一种常见的急性胃损伤疾病。目前临床应用的药物不仅不能从根本上治疗胃损伤，而且还具有严重的副作用。因此，迫切需要发现一种温和的药物来治疗酒精性胃溃疡。在此，我们获得了源于焦白术（CAM）的绿色碳点（CAM-CDs），并已证明其在抑制率高达 60%的情况下可安全有效地缓解酒精性胃溃疡。CAM-CDs 通过组织病理学检查可明显减轻胃黏膜损伤，如黏膜缺损、出血和炎性细胞浸润。血清和组织炎性细胞因子的测量以及免疫组织化学结果表明，其胃黏膜保护机制可能涉及通过调节 NF-κB/NLRP3 轴的炎症信号通路降低 IL-1β 和 TNF-α 水平，以及升高 IL-10 水平。CAM-CDs 还可以降低氧化应激标志物（MDA），增加 PGE2 和粘蛋白分泌（MUC5AC），同时对氢钾 ATP 酶和胃蛋白酶活性有轻微抑制作用，从而保护胃黏膜，并增加胃溃疡大鼠肠道微生物群的微生物多样性和调节物种组成。我们的工作为利用基于碳的纳米材料开发新型温和药物提供了新的视角。
