Neurological Disorders Research Center, Qatar Biomedical Research Institute, Hamad Bin Khalifa University, Qatar Foundation, Doha, Qatar.
Core Labs, Hamad Bin Khalifa University, Qatar Foundation, Doha, Qatar.
Micron. 2023 Oct;173:103517. doi: 10.1016/j.micron.2023.103517. Epub 2023 Jul 20.
Transmission electron microscopy (TEM) is a powerful technique that enables visualization of structural details inside cells. Prior to TEM imaging, biological samples must undergo several preparation steps that are optimized according to the sample type. Currently, there are limited protocols for the preparation of blood samples for TEM imaging. Here, we provide a detailed step-by-step method for preparing blood samples for TEM imaging. This protocol enables robust visualization of the ultrastructures of blood immune cells. In addition, we describe the typical cellular features that can be used to distinguish between different immune cells in the blood, such as neutrophils, eosinophils, monocytes, and lymphocytes. This protocol is useful for studying ultrastructural changes in blood immune cells under various physiological and disease conditions.
透射电子显微镜（TEM）是一种强大的技术，可实现细胞内部结构细节的可视化。在 TEM 成像之前，生物样本必须经过几个根据样本类型进行优化的准备步骤。目前，用于 TEM 成像的血液样本制备方法有限。在这里，我们提供了一种用于 TEM 成像的血液样本准备的详细分步方法。该方案可实现血液免疫细胞超微结构的稳健可视化。此外，我们还描述了可用于区分血液中不同免疫细胞（如中性粒细胞、嗜酸性粒细胞、单核细胞和淋巴细胞）的典型细胞特征。该方案可用于研究各种生理和疾病条件下血液免疫细胞的超微结构变化。
