Cook J L, Shaffer J B, Bewley G C, MacIntyre R J, Wright D A
J Biol Chem. 1986 Sep 5;261(25):11751-5.
A genomic clone containing Drosophila sn-glycerol-3-phosphate dehydrogenase sequences has been isolated using a mixture of synthetic tridecanucleotides as a hybridization probe. The clone as well as the synthetic probe mixture was found to hybridize to an abundant poly(A)+ RNA of 1700 bases. A partial DNA sequence obtained for a 40-amino acid region containing the oligonucleotide hybridization site was found to agree with the known Drosophila protein sequence data for this region of the protein. In situ hybridization of this clone to the polytene chromosomes of wild type flies and flies bearing chromosomal aberrations that delimit the Gpdh+ locus have allowed us to decisively place the gene in the distal region of 26A on the left arm of the second chromosome.
利用合成的十三聚核苷酸混合物作为杂交探针，已分离出一个含有果蝇甘油-3-磷酸脱氢酶序列的基因组克隆。发现该克隆以及合成探针混合物能与一种1700个碱基的丰富的聚腺苷酸加尾（poly(A)+）RNA杂交。对包含寡核苷酸杂交位点的一个40个氨基酸区域获得的部分DNA序列，发现与该蛋白质这一区域已知的果蝇蛋白质序列数据相符。将此克隆与野生型果蝇以及带有界定Gpdh+基因座的染色体畸变的果蝇的多线染色体进行原位杂交，使我们能够明确地将该基因定位在第二条染色体左臂26A的远端区域。
