Koga A, Baba H, Kusakabe S, Hattori M, Mukai T
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
J Mol Evol. 1993 Jun;36(6):532-5. doi: 10.1007/BF00556357.
The glycerol-3-phosphate dehydrogenase (GPDH, E.C. 1.1.1.8) gene of Drosophila melanogaster contains a tandem duplication of a 4.5-kb-long DNA fragment. Survey of the Gpdh gene region by the Southern blot analysis revealed the following features of this gene duplication: (1) The duplication was not observed in chromosome lines that carry In(2L)t, a cosmopolitan chromosomal inversion in this species. The duplication and the inversion are in linkage disequilibrium. (2) The duplication is polymorphic in the Japan and US natural populations examined. Its frequency is 0.26 on an average in In(2L)t-free chromosomes. (3) Triplication is absent or has not become frequent in the populations surveyed. Possible evolutionary factors of this duplication polymorphism are discussed.
黑腹果蝇的甘油 - 3 - 磷酸脱氢酶（GPDH，E.C. 1.1.1.8）基因包含一个4.5千碱基对长的DNA片段的串联重复。通过Southern印迹分析对Gpdh基因区域进行的调查揭示了这种基因重复的以下特征：（1）在携带In(2L)t（该物种中一种常见的染色体倒位）的染色体品系中未观察到重复。重复和倒位处于连锁不平衡状态。（2）在所检测的日本和美国自然种群中，该重复是多态性的。在不含In(2L)t的染色体上，其频率平均为0.26。（3）在所调查的种群中不存在三倍体或三倍体尚未变得常见。讨论了这种重复多态性可能的进化因素。
