Department of Yunnan Tumor Research Institute, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Kunming, 650118, China.
Ganzhou Cancer Hospital, Ganzhou, 341000, China.
BMC Cancer. 2023 Aug 9;23(1):735. doi: 10.1186/s12885-023-11233-2.
The centromere protein O (CENPO) is an important member of the centromere protein family. However, the role of CENPO in pan-cancer and immune infiltration has not been reported. Here, we investigated the role of CENPO in pan-cancer and further validated its role in lung adenocarcinoma (LUAD) by in vitro experiments.
The UCSC Xena database and The Cancer Genome Atlas (TCGA)-LUAD data were used to assess the expression levels of CENPO. The potential value of CENPO as a diagnostic and prognostic biomarker for pan-cancer was evaluated using TCGA data and the GEPIA database. The -expression profiles of LUAD patients and the corresponding clinical data were downloaded for correlation analysis. The role of CENPO in immune infiltration was investigated using the UCSC Xena database. Subsequently, qRT-PCR was performed to detect the expression of CENPO. Cell proliferation, migration, and invasion were determined using CCK-8, wound-healing assay, and transwell assay, respectively.
CENPO is highly expressed in most cancers, and the upregulation of CENPO is associated with poor prognosis in many cancers. CENPO expression correlates with age, TNM stage, N stage, T stage, and receipt of radiotherapy in LUAD patients, and LUAD patients with high CENPO expression have poorer overall survival (OS) and disease-free survival (DFS). In addition, CENPO expression is associated with immune cell infiltration and immune checkpoint inhibitors. Moreover, the expression of CENPO was closely related to the expression of tumor mutational load and microsatellite instability. In vitro experiments showed that CENPO expression was increased in LUAD cell lines and that knockdown of CENPO significantly inhibited the proliferation, cell invasion, and migration ability of LUAD cells.
CENPO may be a potential pan-cancer biomarker and oncogene, especially in LUAD. In addition, CENPO is associated with immune cell infiltration and may serve as a new molecular therapeutic target and effective prognostic marker for LUAD.
着丝粒蛋白 O（CENPO）是着丝粒蛋白家族的重要成员。然而，CENPO 在泛癌和免疫浸润中的作用尚未报道。在这里，我们研究了 CENPO 在泛癌中的作用，并通过体外实验进一步验证了其在肺腺癌（LUAD）中的作用。
使用 UCSC Xena 数据库和癌症基因组图谱（TCGA）-LUAD 数据评估 CENPO 的表达水平。使用 TCGA 数据和 GEPIA 数据库评估 CENPO 作为泛癌诊断和预后生物标志物的潜在价值。下载 LUAD 患者的 -表达谱和相应的临床数据进行相关性分析。使用 UCSC Xena 数据库研究 CENPO 在免疫浸润中的作用。随后，进行 qRT-PCR 检测 CENPO 的表达。使用 CCK-8 法、划痕愈合实验和 Transwell 实验分别检测细胞增殖、迁移和侵袭能力。
CENPO 在大多数癌症中高表达，CENPO 的上调与许多癌症的不良预后相关。在 LUAD 患者中，CENPO 的表达与年龄、TNM 分期、N 分期、T 分期和接受放疗有关，CENPO 高表达的 LUAD 患者总生存期（OS）和无病生存期（DFS）更差。此外，CENPO 的表达与免疫细胞浸润和免疫检查点抑制剂相关。此外，CENPO 的表达与肿瘤突变负荷和微卫星不稳定性密切相关。体外实验表明，LUAD 细胞系中 CENPO 的表达增加，敲低 CENPO 显著抑制 LUAD 细胞的增殖、细胞侵袭和迁移能力。
CENPO 可能是一种潜在的泛癌生物标志物和癌基因，特别是在 LUAD 中。此外，CENPO 与免疫细胞浸润有关，可能成为 LUAD 的新的分子治疗靶点和有效的预后标志物。
