Rezaei Larijani Saemeh, Hosseini Seyed Mohammad, Ekrami Behrang
Department of Pathology, Babol Branch, Islamic Azad University, Babol, Iran.
Department of Animal Science, Chaloos Branch, Islamic Azad University, Chaloos, Iran.
Int J Reprod Biomed. 2023 Jul 24;21(6):499-508. doi: 10.18502/ijrm.v21i6.13637. eCollection 2023 Jun.
Zinc oxide nanoparticles (ZnO-NPS) are widely used inhuman life; however, they do have side effects on human health.
This study aimed to evaluatethedifferent doses of ZnO-NPS on testicular tissue.
35 male Wistar rats(10-12 wk, 220 20gr) were divided into 7 groups of 5, including the control group (gavaged distilled water daily), sham group (received intraperitoneal doses of distilled water twice a week). The group receivedintraperitoneal ZnO-NPS (25, 50, and 100 mg/kg body weight, twice a week), and gavage (150 and 200 mg/kg body weight daily). All stages of the test were performed in 4 wk then serum testosterone and tissue malondialdehyde, and ferric reducing antioxidant power levels were measured, also testes histopathological evaluation was performed.
Our results showed that a reduced cell population of spermatozoa was observed in the group that received 25 mg/kg ZnO-NPS, while a reduced cell population of spermatozoa, edema, hyperemia, and vacuolar degeneration were observed in the group that received 50 and 100 mg/kg ZnO-NPS. The maximum amount of lesions were observed in the dose of 200 mg/kg. The highest amount of ferric reducing antioxidant power and testosterone levels were observed in the control group. Also, a 100 mg/kg intraperitoneal dose of ZnO-NPS and 150 mg/kg oral dose of ZnO-NPS were suitable doses to create a model of male genital lesions.
Nanoparticles are harmful factors for the reproductive system and consequently affect infertility, which requires the toxicity of the concentration of these nanoparticles to be evaluated and controlled.
氧化锌纳米颗粒（ZnO-NPs）在人类生活中广泛使用；然而，它们确实对人类健康有副作用。
本研究旨在评估不同剂量的ZnO-NPs对睾丸组织的影响。
将35只雄性Wistar大鼠（10 - 12周龄，体重220±20克）分为7组，每组5只，包括对照组（每日灌胃蒸馏水）、假手术组（每周两次腹腔注射蒸馏水）。其余组每周两次腹腔注射ZnO-NPs（25、50和100毫克/千克体重），并每日灌胃（150和200毫克/千克体重）。试验的所有阶段均在4周内进行，然后测量血清睾酮、组织丙二醛和铁还原抗氧化能力水平，同时进行睾丸组织病理学评估。
我们的结果表明，接受25毫克/千克ZnO-NPs的组中观察到精子细胞数量减少；而接受50和100毫克/千克ZnO-NPs的组中观察到精子细胞数量减少、水肿、充血和空泡变性。在200毫克/千克剂量组中观察到的损伤最多。对照组中观察到最高水平的铁还原抗氧化能力和睾酮水平。此外，100毫克/千克腹腔注射剂量的ZnO-NPs和150毫克/千克口服剂量的ZnO-NPs是建立男性生殖器损伤模型的合适剂量。
纳米颗粒是生殖系统的有害因素，因此会影响不育症，这需要对这些纳米颗粒浓度的毒性进行评估和控制。
