Clin Lab. 2023 Aug 1;69(8). doi: 10.7754/Clin.Lab.2023.220803.
The Clinical and Laboratory Standards Institute recommends rejecting hemolyzed samples for coagulation tests. Sysmex CS5100 analyzer using an optical method is commonly used in laboratories. The influence of hemolysis on coagulation test has rarely been studied when tested on Sysmex CS5100. Determining this influence is necessary.
Freshly collected samples were artificially hemolyzed to simulate the hemolysis processes. Coagulation tests were conducted on a Sysmex CS5100 coagulation analyzer. Detection values before and after hemolysis were compared.
The results showed that after hemolysis detection, the prothrombin time (PT) statistically decreased, while the partial thromboplastin time (APTT) statistically increased. There were no significant differences in fibrinogen (Fg), thrombin time (TT), D-dimer (DD) or fibrinogen degradation products (FDPs). Antithrombin activity was elevated in hemolyzed samples.
Although differences in PT and APTT were statistically significant, there was no need for rejection of hemolyzed samples due to insufficient clinical effects when tested on Sysmex CS5100 analyzer. Falsely elevated AT result may lead to misdiagnosis in patients with severe diseases, which should be carefully considered.
临床和实验室标准协会建议拒收用于凝血检测的溶血样本。Sysmex CS5100 分析仪采用光学方法，在实验室中被广泛应用。Sysmex CS5100 上检测溶血对凝血检测的影响的相关研究较少，确定这种影响是必要的。
采集新鲜样本进行人工溶血，模拟溶血过程。在 Sysmex CS5100 凝血分析仪上进行凝血检测。比较溶血前后的检测值。
结果显示，溶血后凝血酶原时间（PT）有统计学意义的降低，而部分凝血活酶时间（APTT）有统计学意义的升高。纤维蛋白原（Fg）、凝血酶时间（TT）、D-二聚体（DD）或纤维蛋白（原）降解产物（FDPs）无显著差异。抗凝血酶活性在溶血样本中升高。
尽管 PT 和 APTT 的差异具有统计学意义，但在 Sysmex CS5100 分析仪上检测时，由于临床效果不足，无需拒收溶血样本。严重疾病患者中， falsely elevated AT 结果可能导致误诊，应谨慎考虑。
