Department of Biological Sciences and Biotechnology, Hannam University Jeonmin-dong, Yuseong-gu, Daejeon 34430, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Aug;73(8). doi: 10.1099/ijsem.0.006001.
A Gram-stain-negative, rod-shaped, polar flagellated, aerobic, light-yellow bacterium, designated as 2012CJ41-6, was isolated from a sponge sample of from Chuja-myeon, Jeju-si, Jeju-do, Republic of Korea. On the basis of 16S rRNA gene sequencing, strain 2012CJ41-6 clustered with species of the genus and appeared closely related to . DSM 27839 (96.46 % similarity), . CECT 4357 (96.32 %), . ZGT108 (96.32 %), CECT 7639 (96.32 %) and . CECT 4292 (96.16 %). The average nucleotide identity and digital DNA-DNA hybridization between strain 2012CJ41-6 and the most closely related strain was 75.3 % and 19.6 %, indicating that 2012CJ41-6 represents a novel species of the genus . Growth occurred at 15-37 °C on marine medium in the presence of 0.5-10 % (w/v) NaCl and at pH 5.5-8.5. The DNA G+C content of the genomic DNA was 60.80 mol%, and ubiquinone-10 (Q-10) was the major respiratory quinone. The major cellular fatty acids (>5 %) were C 7 and/or C 6 (summed feature 8). The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, one unidentified phospholipid, one unidentified aminolipid, one unidentified aminophospholipid and five unidentified lipids. Physiological and biochemical characteristics indicated that strain 2012CJ41-6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 2012CJ41-6 (=KACC 22645=LMG 32585).
一株革兰氏阴性、杆状、极生鞭毛、需氧、浅黄色的细菌，被命名为 2012CJ41-6，从韩国济州岛楚江面的海绵样本中分离得到。基于 16S rRNA 基因测序，菌株 2012CJ41-6 与属内的种聚在一起，与 最为密切相关。DSM 27839（96.46%相似度）、CECT 4357（96.32%相似度）、ZGT108（96.32%相似度）、CECT 7639（96.32%相似度）和 CECT 4292（96.16%相似度）。菌株 2012CJ41-6 与最密切相关菌株的平均核苷酸同一性和数字 DNA-DNA 杂交率分别为 75.3%和 19.6%，表明 2012CJ41-6 代表了属的一个新种。在海洋培养基中，该菌在 15-37°C、0.5-10%（w/v）NaCl 和 pH5.5-8.5 的条件下生长。基因组 DNA 的 G+C 含量为 60.80mol%，主要呼吸醌为泛醌-10（Q-10）。主要细胞脂肪酸（>5%）为 C7 和/或 C6（特征 8 总和）。极性脂类由磷脂酰甘油、双磷脂酰甘油、磷脂酰胆碱、一种未鉴定的磷脂、一种未鉴定的胺脂、一种未鉴定的氨基磷脂和五种未鉴定的脂类组成。生理生化特性表明，菌株 2012CJ41-6 代表了属的一个新种，建议将其命名为 sp. nov.。模式菌株为 2012CJ41-6（=KACC 22645=LMG 32585）。
