Department of Biological Sciences and Biotechnology, Hannam University Jeonmin-dong, Yuseong-gu, Daejeon 34430, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005136.
A Gram-stain-negative, rod-shaped, motile via polar flagellum, facultatively aerobic, light-yellow, bacterium (designated 188UL20-2) was isolated from a mussel sample of collected on Ulleung Island, Ulleung-gun, Gyeongsangbuk-do, Republic of Korea. On the basis of 16S rRNA gene sequencing results, strain 188UL20-2 clustered with species of the genus and appeared closely related to DSM 23086 (96.59%), DSM 26147 (96.57%), DSM 14398 (96.37%) and DSM 17657 (95.97%). The average nucleotide identity and digital DNA-DNA hybridization values between strain 188UL20-2 and its closest related strain were 71.3 and 16.4%, indicating that 188UL20-2 represents a novel species of the genus . Growth occurred at 18-37 °C on MA medium in the presence of 1-4% NaCl (w/v) and at pH 5.0-10.0. The DNA G+C content of the genomic DNA was 45.4 mol%, and ubiquinone-8 (Q-8) was the major respiratory quinone. The major cellular fatty acids (>5%) were C 6 and/or C 7 (summed feature 3), C 7 and/or C 6 (summed feature 8), C, C iso, C, C iso and C. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, unidentified aminophospholipid, unidentified glycolipid and seven unidentified lipids. Physiological and biochemical characteristics indicated that strain 188UL20-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 188UL20-2 (=KACC 22258=LMG 32202).
一株革兰氏阴性、杆状、通过极生鞭毛运动、兼性需氧、浅黄色的细菌（命名为 188UL20-2）从韩国庆尚北道郁陵郡郁陵岛采集的贻贝样本中分离得到。基于 16S rRNA 基因测序结果，菌株 188UL20-2 与属内的物种聚在一起，与 DSM 23086（96.59%）、DSM 26147（96.57%）、DSM 14398（96.37%）和 DSM 17657（95.97%）最为接近。菌株 188UL20-2 与其最接近的菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 71.3%和 16.4%，表明 188UL20-2 代表了属内的一个新种。在 MA 培养基中，在 1-4%（w/v）NaCl 存在的情况下，温度为 18-37°C，pH 值为 5.0-10.0 时，可生长。基因组 DNA 的 G+C 含量为 45.4mol%，主要呼吸醌为泛醌-8（Q-8）。主要的细胞脂肪酸（>5%）为 C 6 和/或 C 7（总和特征 3）、C 7 和/或 C 6（总和特征 8）、C 16:0、C 17:0 iso 和 C 18:1ω7c。极性脂由磷脂酰甘油、磷脂酰乙醇胺、两种未鉴定的磷脂、未鉴定的氨基磷脂、未鉴定的糖脂和七种未鉴定的脂组成。生理生化特征表明，菌株 188UL20-2 代表了属内的一个新种，提议将其命名为 sp. nov.。模式菌株为 188UL20-2（=KACC 22258=LMG 32202）。
