Preparation of room-temperature phosphorescence-ratiometric fluorescence magnetic mesoporous imprinted microspheres and its application in detection of malachite green and tartrazine in multimatrix.

The photoluminescent properties of Mn-doped ZnS quantum dots were fully exploited, and room-temperature phosphorescence (RTP)-ratiometric fluorescence (RF) magnetic mesoporous molecularly imprinted polymers (PFMM-MIPs) were prepared by integrating molecular imprinting technology. RTP was used to detect malachite green (MG). The fluorescence at 420 nm and the peak at 590 nm in the fluorescence mode were used as the response reference signals respectively to detect tartrazine (TZ). The linear responsive range and detection limit of MG were 0.01-150 μM and 4.3 nM, and these of TZ were 0.05-80 μM and 23.7 nM. RTP, which can avoid the interference of background fluorescence, and RF with self-calibration ability can both largely weaken the matrix effect. This work enables single-probe-type MIPs to achieve dual-target analysis via RTP and RF. This method provides excellent sensitivity, specificity, recovery and recyclability, and is expected to be prospectively applied in the fields of food, environment and biological analyses.