Arslan Taner, Güney Orhan
Istanbul Technical University, Departments of Chemistry, 34469, Maslak, Istanbul, Turkey.
Istanbul Technical University, Departments of Chemistry, 34469, Maslak, Istanbul, Turkey.
Anal Biochem. 2020 Feb 15;591:113540. doi: 10.1016/j.ab.2019.113540. Epub 2019 Dec 13.
A ratiometrically responsive sensor for dsDNA is reported, based on molecularly imprinted polymer coated quantum dots (MIP-QDs). A new platform is described for probing dsDNA by tracing the "turn on" fluorescence signal of malachite green (MG) as a cationic dye and "turn off-on" room temperature phosphorescence (RTP) signal of MIP-QDs/MG nanohybrids. The interaction between MIP-QDs surface and MG discloses an intense quenching in RTP (turning off) by a phosphorescence resonance energy transfer (PRET) process. After the addition of dsDNA, MG molecules escape from the MIP-QDs surface and intercalate into the dsDNA, resulting in the restoration of RTP intensity of MIP-QDs (turning on) and also enhancing in fluorescence of MG. This outcome hereby can be employed for the selective sensing of dsDNA via optical response. The ratio of fluorescence enhancement of MG to RTP intensity of MIP-QDs is proportional to the concentration of dsDNA in the range of 0.089-1.79 μg/mL with a detection limit (3σ/K) of 19.48 ng/mL under the optimized experimental conditions.
报道了一种基于分子印迹聚合物包覆量子点（MIP-QDs）的用于双链DNA的比率响应传感器。描述了一种新平台，通过追踪孔雀石绿（MG）作为阳离子染料的“开启”荧光信号以及MIP-QDs/MG纳米杂化物的“关闭-开启”室温磷光（RTP）信号来探测双链DNA。MIP-QDs表面与MG之间的相互作用通过磷光共振能量转移（PRET）过程揭示了RTP的强烈猝灭（关闭）。加入双链DNA后，MG分子从MIP-QDs表面逸出并插入双链DNA中，导致MIP-QDs的RTP强度恢复（开启），同时MG的荧光增强。因此，这一结果可用于通过光学响应选择性传感双链DNA。在优化的实验条件下，MG荧光增强与MIP-QDs的RTP强度之比与双链DNA浓度在0.089-1.79μg/mL范围内成正比，检测限（3σ/K）为19.48ng/mL。
