Shao Yanming, Zhao Zhizhen, An Jun, Hao Caifeng, Kang Mengyi, Rong Xuan, Zhao Huanhuan, Feng Huanran
College of Chemistry and Chemical Engineering, Key Laboratory of Auxiliary Chemistry and Technology for Chemical Industry, Ministry of Education, Shaanxi Key Laboratory of Chemical Additives for Industry, Shaanxi University of Science and Technology, Xi'an, Shaanxi, 710021, People's Republic of China.
Interdisciplinary Research Center of Smart Sensors, Shaanxi Key Laboratory of High-Orbits-Electron Materials and Protection Technology for Aerospace, School of Advanced Materials and Nanotechnology, Xidian University, Shaanxi, 710126, People's Republic of China.
Mikrochim Acta. 2024 Feb 26;191(3):156. doi: 10.1007/s00604-024-06244-0.
A magnetic fluorescent molecularly imprinted sensor was successfully prepared and implemented to determine catechol (CT). FeO nanoparticles were synthesized by the solvothermal technique and mesoporous FeO@SiO@mSiO imprinted carriers were prepared by coating nonporous and mesoporous SiO shells on the surface of the FeO subsequently. The magnetic surface molecularly imprinted fluorescent sensor was created after the magnetic mesoporous carriers were modified with γ-methacryloxyl propyl trimethoxy silane to introduce double bonds on the surface of the carries and the polymerization was carried out in the presence of CT and fluorescent monomers. The magnetic mesoporous carriers were modified with γ-methacryloxyl propyl trimethoxy silane and double bonds were introduced on the surface of the carriers. After CT binding with the molecularly imprinted polymers (MIPs), the fluorescent intensity of the molecularly imprinted polymers (E = 400 nm, E = 523 nm) increased significantly. The fluorescent intensity ratio (F/F) of the sensor demonstrated a favorable linear correlation with the concentration of CT between 5 and 50 μM with a detection limit of 0.025 μM. Furthermore, the sensor was successfully applied to determine CT in actual samples with recoveries of 96.4-105% and relative standard deviations were lower than 3.5%. The results indicated that the research of our present work provided an efficient approach for swiftly and accurately determining organic pollutant in water.
成功制备了一种磁性荧光分子印迹传感器并将其用于测定邻苯二酚（CT）。通过溶剂热技术合成了FeO纳米颗粒，随后在FeO表面包覆无孔和介孔SiO壳层，制备了介孔FeO@SiO@mSiO印迹载体。在用γ-甲基丙烯酰氧基丙基三甲氧基硅烷对磁性介孔载体进行改性以在载体表面引入双键后，在CT和荧光单体存在的情况下进行聚合反应，从而制备了磁性表面分子印迹荧光传感器。用γ-甲基丙烯酰氧基丙基三甲氧基硅烷对磁性介孔载体进行改性，在载体表面引入双键。CT与分子印迹聚合物（MIP）结合后，分子印迹聚合物的荧光强度（激发波长 = 400 nm，发射波长 = 523 nm）显著增加。该传感器的荧光强度比（F/F）与5至50 μM的CT浓度呈现出良好的线性关系，检测限为0.025 μM。此外，该传感器成功应用于实际样品中CT的测定，回收率为96.4 - 105%，相对标准偏差低于3.5%。结果表明，我们目前的工作研究为快速、准确地测定水中有机污染物提供了一种有效的方法。
