Giles H G, Meggiorini S, Vidins E I
Can J Physiol Pharmacol. 1986 Jun;64(6):717-9. doi: 10.1139/y86-120.
Plasma samples (0.5 mL) were analyzed for ethanol and acetate by head space gas chromatography using a Porapak QS column (80-100 mesh). Acetate was esterified to methyl acetate simply by the addition of acidified methanol. The analytical ranges were 1.61-103 and 0.05-1.9 mM for ethanol and acetate, respectively. The within-run coefficients of variation did not exceed 4.7% for acetate and 2.7% for ethanol. After the oral administration of ethanol to two healthy human subjects, the concentration versus time profiles of plasma ethanol and acetate were determined. Acetate concentrations (0.4-0.9 mM) remained quite constant while ethanol was being metabolized and appeared not to be affected by the concentration of ethanol in the range 3-18 mM. The advantages of the method are speed and simplicity.
使用Porapak QS柱（80 - 100目）通过顶空气相色谱法分析0.5 mL血浆样品中的乙醇和乙酸盐。仅通过添加酸化甲醇，乙酸盐就被酯化生成乙酸甲酯。乙醇和乙酸盐的分析范围分别为1.61 - 103 mM和0.05 - 1.9 mM。乙酸盐的批内变异系数不超过4.7%，乙醇的批内变异系数不超过2.7%。在向两名健康人体受试者口服乙醇后，测定了血浆乙醇和乙酸盐的浓度-时间曲线。在乙醇代谢过程中，乙酸盐浓度（0.4 - 0.9 mM）保持相当恒定，且在3 - 18 mM的乙醇浓度范围内似乎不受影响。该方法的优点是速度快且操作简单。
