Bruno R, Santoni Y, Botta A, Cano J P
Toxicol Eur Res. 1982 Jan;4(1):31-4.
Plasma acetate is extracted using ethanol in alkaline medium, in presence of internal standard. After concentration of the hydroalcoholic phase, the chromatographic analysis is performed utilizing 10% SP 1200 and 1% H3PO4 on chromosorb W. The linearity of the method has been tested from 0.05 to 10 mM/I, its reproducibility is +/- 3.5% for levels ranging from 0.1 to 10 mM/I and +/- 10% for those smaller than 0.1 mM/I. This method is sensitive enough to assess the physiological acetate levels and those obtained after ethanol biotransformation.
在存在内标的情况下，于碱性介质中用乙醇提取血浆乙酸盐。浓缩水醇相后，利用10%的SP 1200和1%的磷酸在Chromosorb W上进行色谱分析。该方法的线性已在0.05至10 mM/I范围内进行测试，对于0.1至10 mM/I的水平，其重现性为±3.5%，对于小于0.1 mM/I的水平，重现性为±10%。该方法灵敏度足以评估生理乙酸盐水平以及乙醇生物转化后获得的乙酸盐水平。
