顶空气相色谱法测定人血液和尿液中的游离及结合乙醇、乙醛和乙酸盐。

Determinations of free and bound ethanol, acetaldehyde, and acetate in human blood and urine by headspace gas chromatography.

作者信息

Tsukamoto S, Kanegae T, Isobe E, Hirose M, Nagoya T

机构信息

Department of Legal Medicine, Nihon University School of Medicine, Tokyo, Japan.

出版信息

Nihon Arukoru Yakubutsu Igakkai Zasshi. 1998 Jun;33(3):200-9.

PMID:9701997

Abstract

The improved PCA method leads to accurate measurement of ethanol, acetaldehyde, and acetate in blood and urine by headspace gas chromatography. It is important to prevent the formation of artifactual acetaldehyde from coexistent ethanol. The column used for detection of alcohol metabolites was the fused silica glass capillary column bonded with PEG-20M or the fused silica glass capillary column of Pora PLOT Q. In bound alcohol metabolites, recent measurements of hemoglobin-associated acetaldehyde in blood, and ethanol conjugate and acetaldehyde conjugate in urine are reviewed and described as a marker of alcohol abuse.

摘要

改进的主成分分析（PCA）方法可通过顶空气相色谱法准确测量血液和尿液中的乙醇、乙醛和乙酸盐。防止共存乙醇产生人为乙醛至关重要。用于检测酒精代谢物的柱是键合有聚乙二醇-20M的熔融石英玻璃毛细管柱或Pora PLOT Q熔融石英玻璃毛细管柱。在结合的酒精代谢物方面，对近期血液中血红蛋白相关乙醛以及尿液中乙醇共轭物和乙醛共轭物的测量进行了综述，并将其描述为酒精滥用的标志物。