Law Ilya, Becker Erin, Spoja Brandon S, Kobal Katrina, Yiridoe Martha, Alashraf Abdul, Parker Beth L, McCarthy David T, Murphy Heather M
Water, Health and Applied Microbiology Lab (WHAM Lab), Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Morwick G360 Groundwater Research Institute, College of Engineering and Physical Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
ACS ES T Water. 2025 Mar 25;5(4):1673-1682. doi: 10.1021/acsestwater.4c01029. eCollection 2025 Apr 11.
Passive sampling has shown promise as an alternative approach for monitoring of pathogens in aquatic matrices. We conducted two controlled experiments to compare the efficacy of membrane passive sampling to composite sampling in both wastewater and surface water for the detection of and . We also investigated the relative uptake of and onto membrane passive samplers over time. Both sampling methods returned positive detections of at all deployment times (4, 8, 24, 48, 72, and 96 h) in both water matrices. Passive sampling for showed similar detection rates as composite samples in surface water (31% passive; 41% composite) and wastewater (76% passive; 86% composite). We found significant linear uptake of onto passive samplers up to 96 h in surface water ( = 0.932; = 0.002). In wastewater, maximum passive sampler uptake of was reached after 24 h. For , linear uptake was observed up to 96 h for both surface water ( = 0.805; = 0.015) and wastewater ( = 0.877; = 0.006). Our results support that membrane passive samplers may be used for the detection of and in surface waters for up to 96 h.
被动采样已显示出有望成为监测水生环境中病原体的一种替代方法。我们进行了两项对照实验，以比较膜被动采样与混合采样在废水和地表水中检测[具体物质1]和[具体物质2]的效果。我们还研究了随着时间推移，[具体物质1]和[具体物质2]在膜被动采样器上的相对吸附情况。在两种水体基质中，两种采样方法在所有部署时间（4、8、24、48、72和96小时）均检测到了[具体物质1]呈阳性。对于[具体物质1]的被动采样，在地表水（被动采样31%；混合采样41%）和废水中（被动采样76%；混合采样86%）显示出与混合样品相似的检测率。我们发现在地表水中，直到96小时，[具体物质1]在被动采样器上有显著的线性吸附（R² = 0.932；p = 0.002）。在废水中，被动采样器对[具体物质1]的最大吸附在24小时后达到。对于[具体物质2]，在地表水（R² = 0.805；p = 0.015）和废水（R² = 0.877；p = 0.006）中均观察到直到96小时的线性吸附。我们的结果支持膜被动采样器可用于检测地表水中的[具体物质1]和[具体物质2]长达96小时。
