Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
J Nat Prod. 2023 Aug 25;86(8):2046-2053. doi: 10.1021/acs.jnatprod.3c00609. Epub 2023 Aug 11.
Depsidones are significant in structural diversity and broad in biological activities; however, their biosynthetic pathways have not been well understood and have attracted considerable attention. Herein, we heterologously reconstituted a depsidone encoding gene cluster from sp. SCSIO SY280D in A1145, leading to production of mollicellins, a representative family of depsidones, and discovering a bifunctional P450 monooxygenase that catalyzes both ether formation and hydroxylation in the biosynthesis of the mollicellins. The functions of a decarboxylase and an aromatic prenyltransferase are also characterized to understand the tailoring modification steps. This work provides important insights into the biosynthesis of mollicellins.
漆木酮具有显著的结构多样性和广泛的生物活性，但它们的生物合成途径尚未得到很好的理解，因此引起了相当大的关注。本文中，我们在 A1145 中异源重建了来自 sp. SCSIO SY280D 的漆木酮编码基因簇，导致了 mollicellins 的产生，mollicellins 是漆木酮的一个代表性家族，并发现了一种双功能 P450 单加氧酶，它在 mollicellins 的生物合成中催化醚形成和羟化。还对脱羧酶和芳基 prenyltransferase 的功能进行了表征，以了解修饰步骤。这项工作为 mollicellins 的生物合成提供了重要的见解。
