College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an City 271018, Shandong Province, China; Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, College of Basic Medical Sciences, Shandong First Medical University, Tai'an City 271000, Shandong Province, China.
Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an City 271018, Shandong Province, China.
Poult Sci. 2023 Oct;102(10):102969. doi: 10.1016/j.psj.2023.102969. Epub 2023 Jul 26.
Since 2005, novel duck reoviruses have been outbreaks in duck breeding areas such as central China and South China. In recent years, the incidence rate of this disease is still increasing, bringing serious economic losses to waterfowl breeding industry. This study isolated 3 novel duck reoviruses (NDRV-SDLS, NDRV-SDWF, and NDRV-SDYC) from sick ducks in 3 local duck farms in Shandong Province. The study aimed to investigate the characteristics of these viruses. The virus is inoculated into duck embryo fibroblasts, where the virus replicates to produce syncytium and dies within 3 to 5 d. The viruses were also isolated from infected ducks, and RT-PCR amplified the whole genomes after passage purification in duck embryos. The resulting whole genome was analyzed for genetic evolution. The total length of the gene sequencing was 23,418 bp, divided into 10 fragments. Gene sequence comparison showed that the 3 strains had high similarity with novel duck reoviruses (NDRV) but low similarity with chicken-origin reovirus (chicken ARV) and Muscovy duck reovirus (MDRV), especially in the σC segment. Phylogenetic analysis of the 10 fragments showed that the 3 isolates constituted the same evolutionary clade as other DRV reference strains and were far related to ARV and MDRV in different evolutionary clades. The results of all 10 segments indicate that the isolates are in the evolutionary branch of NDRV, suggesting that the novel waterfowl reovirus is the dominant circulating strain in Shandong. This study complements the gene bank information of NDRV and provides references for vaccine research and disease prediction of NDRV in Shandong.
自 2005 年以来，新型鸭呼肠孤病毒已在中国中部和南部等养鸭区爆发。近年来，该病的发病率仍在上升，给水禽养殖业带来了严重的经济损失。本研究从山东省 3 个当地养鸭场的病鸭中分离出 3 株新型鸭呼肠孤病毒（NDRV-SDLS、NDRV-SDWF 和 NDRV-SDYC）。本研究旨在探讨这些病毒的特征。将病毒接种到鸭胚成纤维细胞中，病毒在其中复制产生合胞体，并在 3 至 5 天内死亡。还从感染鸭中分离出病毒，并在鸭胚中传代纯化后通过 RT-PCR 扩增全基因组。对所得全基因组进行遗传进化分析。基因测序的总长度为 23418bp，分为 10 个片段。基因序列比较表明，这 3 株与新型鸭呼肠孤病毒（NDRV）具有高度相似性，但与鸡源呼肠孤病毒（chicken ARV）和麝香鸭呼肠孤病毒（MDRV）的相似性较低，特别是在 σC 片段。10 个片段的系统进化分析表明，这 3 个分离株与其他 DRV 参考株构成相同的进化分支，与 ARV 和 MDRV 处于不同的进化分支。所有 10 个片段的结果表明，分离株处于 NDRV 的进化分支，提示新型水禽呼肠孤病毒是山东流行的优势循环株。本研究补充了 NDRV 的基因库信息，为 NDRV 在山东的疫苗研究和疾病预测提供了参考。
