Peng Zhifeng, Zhang Han, Zhang Xiaozhan, Wang Haiyan, Liu Zihan, Qiao Hongxing, Lv Yujin, Bian Chuanzhou
College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, China.
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.
Front Vet Sci. 2023 Mar 30;10:1137967. doi: 10.3389/fvets.2023.1137967. eCollection 2023.
Novel Duck reovirus (NDRV) is an ongoing non-enveloped virus with ten double-stranded RNA genome segments that belong to the genus , in the family . NDRV-associated spleen swelling, and necrosis disease have caused considerable economic losses to the waterfowl industry worldwide. Since 2017, a significant number of NDRV outbreaks have emerged in China. Herein, we described two cases of duck spleen necrosis disease among ducklings on duck farms in Henan province, central China. Other potential causative agent, including Muscovy duck reovirus (MDRV), Duck hepatitis A virus type 1 (DHAV-1), Duck hepatitis A virus type 3 (DHAV-3), Newcastle disease virus (NDV), and Duck tembusu virus (DTMUV), were excluded by reverse transcription-polymerase chain reaction (RT-PCR), and two NDRV strains, HeNXX-1/2021 and HNJZ-2/2021, were isolated. Sequencing and phylogenetic analysis of the σC genes revealed that both newly identified NDRV isolates were closely related to DRV/SDHZ17/Shandong/2017. The results further showed that Chinese NDRVs had formed two distinct clades, with late 2017 as the turning point, suggesting that Chinese NDRVs have been evolving in different directions. This study identified and genetic characteristics of two NDRV strains in Henan province, China, indicating NDRVs have evolved in different directions in China. This study provides an insight into the ongoing emerged duck spleen necrosis disease and enriches our understanding of the genetic diversity and evolution of NDRVs.
新型鸭呼肠孤病毒（NDRV）是一种无包膜病毒，其基因组由10条双链RNA片段组成，属于呼肠孤病毒科正呼肠孤病毒属。NDRV相关的脾脏肿大和坏死病已给全球水禽产业造成了巨大经济损失。自2017年以来，中国出现了大量NDRV疫情。在此，我们描述了中国中部河南省两个鸭场雏鸭发生鸭脾脏坏死病的病例。通过逆转录-聚合酶链反应（RT-PCR）排除了其他潜在病原体，包括番鸭呼肠孤病毒（MDRV）、鸭甲型肝炎病毒1型（DHAV-1）、鸭甲型肝炎病毒3型（DHAV-3）、新城疫病毒（NDV）和鸭坦布苏病毒（DTMUV），并分离出两株NDRV毒株，即HeNXX-1/2021和HNJZ-2/2021。σC基因的测序和系统发育分析表明，这两株新鉴定的NDRV分离株均与DRV/SDHZ17/山东/2017密切相关。结果进一步表明，中国的NDRV形成了两个不同的进化分支，以2017年末为转折点，这表明中国的NDRV一直在朝着不同方向进化。本研究鉴定了中国河南省两株NDRV毒株及其遗传特征，表明NDRV在中国已朝着不同方向进化。本研究为正在出现的鸭脾脏坏死病提供了见解，并丰富了我们对NDRV遗传多样性和进化的认识。
