Human Phenome Institute, Zhangjiang Fudan International Innovation Center, Fudan University, Shanghai 200438, China.
MOE Key Laboratory of Contemporary Anthropology, Fudan University, Shanghai 200438, China.
Int J Mol Sci. 2023 Jul 26;24(15):11955. doi: 10.3390/ijms241511955.
Kidney stones are a common urological disorder with increasing prevalence worldwide. The treatment of kidney stones mainly relies on surgical procedures or extracorporeal shock wave lithotripsy, which can effectively remove the stones but also result in some complications and recurrence. Therefore, finding a drug or natural compound that can prevent and treat kidney stones is an important research topic. In this study, we aimed to investigate the effects of yellow tea on kidney stone formation and its mechanisms of action. We induced kidney stones in rats by feeding them an ethylene glycol diet and found that yellow tea infusion reduced crystal deposits, inflammation, oxidative stress, and fibrosis in a dose-dependent manner. Through network pharmacology and quantitative structure-activity relationship modeling, we analyzed the interaction network between the compounds in yellow tea and kidney stone-related targets and verified it through in vitro and in vivo experiments. Our results showed that flavonoids in yellow tea could bind directly or indirectly to peroxisome proliferator-activated receptor gamma (PPARG) protein and affect kidney stone formation by regulating PPARG transcription factor activity. In conclusion, yellow tea may act as a potential PPARG agonist for the prevention and treatment of renal oxidative damage and fibrosis caused by kidney stones.
肾结石是一种常见的泌尿系统疾病，全球患病率呈上升趋势。肾结石的治疗主要依赖于手术或体外冲击波碎石术，这些方法虽然可以有效去除结石，但也会导致一些并发症和复发。因此，寻找一种能够预防和治疗肾结石的药物或天然化合物是一个重要的研究课题。在这项研究中，我们旨在探讨黄茶对肾结石形成的影响及其作用机制。我们通过给大鼠喂食乙二醇饮食来诱导肾结石形成，发现黄茶浸液能够以剂量依赖的方式减少晶体沉积、炎症、氧化应激和纤维化。通过网络药理学和定量构效关系建模，我们分析了黄茶中化合物与肾结石相关靶点的相互作用网络，并通过体外和体内实验进行了验证。我们的结果表明，黄茶中的类黄酮可以直接或间接地与过氧化物酶体增殖物激活受体γ（PPARG）蛋白结合，并通过调节 PPARG 转录因子活性来影响肾结石的形成。综上所述，黄茶可能作为一种潜在的 PPARG 激动剂，用于预防和治疗肾结石引起的肾脏氧化损伤和纤维化。
