Department of Biotechnology, Sangmyung University, 20, Hongjimun 2-Gil, Jongno-Gu, Seoul 03016, Republic of Korea.
Fermentation Team, Lotte R&D Center, 210 Magokjungang-Ro, Gangseo-Gu, Seoul 07594, Republic of Korea.
Int J Mol Sci. 2023 Aug 2;24(15):12366. doi: 10.3390/ijms241512366.
Peanut shells (PSs) generated from agricultural waste contain valuable compounds with bioactive properties such as anti-aging, antimicrobial, and antioxidant properties, making it desirable to recycle them as a sustainable resource. The aim of this study is to design an effective luteolin recovery process as the first step of an integrated biorefinery utilizing PSs as raw material. The major extraction variables and their ranges for luteolin recovery from PSs were determined (0-60 °C, 1-5 h, 0-100% MeOH concentration) and a predictive model was derived through a response surface methodology (RSM). Based on the predictive model, the equation determined for the maximal extraction of luteolin at 1 h was as follows: = -1.8475 + 159.57, and the significant range of variables was as follows: 33.8 °C ≤ temperature () ≤ 48.5 °C and 70.0% ≤ MeOH concentration () ≤ 97.5%, respectively. High antioxidant and elastase inhibitory activities of PS extracts were confirmed, and these results support their potential to be used as functional materials. In addition, 39.2% of the solid residue after extraction was carbohydrate, which has potential as a carbon source for fermentation. This study provides a useful direction on an integrated biorefinery approach for sustainable agricultural waste valorization.
从农业废弃物中产生的花生壳（PSs）含有具有生物活性的有价值化合物，如抗衰老、抗菌和抗氧化特性，因此将其回收作为可持续资源是可取的。本研究旨在设计一种有效的木犀草素回收工艺，作为利用 PSs 作为原料的综合生物炼制厂的第一步。确定了从 PSs 中回收木犀草素的主要提取变量及其范围（0-60°C，1-5 小时，0-100%甲醇浓度），并通过响应面法（RSM）得出了预测模型。基于预测模型，在 1 小时内提取木犀草素的最大方程如下： = -1.8475 + 159.57，变量的显著范围如下：33.8°C≤温度（）≤48.5°C 和 70.0%≤甲醇浓度（）≤97.5%，分别。PS 提取物具有高抗氧化和弹性蛋白酶抑制活性，这些结果支持它们作为功能性材料的潜力。此外，提取后固体残渣的 39.2%为碳水化合物，这可能是发酵的碳源。本研究为可持续农业废物增值的综合生物炼制方法提供了有用的方向。
