Oster Nicola, Szewczuk Małgorzata Anna, Zych Sławomir, Stankiewicz Tomasz, Błaszczyk Barbara, Wieczorek-Dąbrowska Marta
Department of Monogastric Animal Science, Faculty of Biotechnology and Animal Husbandry, West Pommeranian University of Technology in Szczecin, 29 Klemensa Janickiego, 71-270 Szczecin, Poland.
Laboratory of Chromatography and Mass Spectroscopy, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology in Szczecin, 29 Klemensa Janickiego, 71-270 Szczecin, Poland.
Animals (Basel). 2023 Jul 31;13(15):2470. doi: 10.3390/ani13152470.
The Janus Kinase 2 (JAK2) tyrosine kinase is an essential component of signal transduction of the class II cytokine receptors, including the growth hormone receptor. Therefore, it may play a crucial role in the signaling pathway of the somatotropic axis, which influences growth, development, and reproductive traits in ruminants. For this purpose, for three breeds of cattle (Hereford, Angus, and Limousin; a total of 781 individuals), two polymorphic sites located in exon 16 (rs210148032; p.Ile704Val, within pseudokinase (JH2)) and exon 23 (silent mutation rs211067160, within JH1 kinase domain) were analyzed. For two breeds of sheep (Pomeranian and Suffolk; 333 individuals in total), two polymorphic sites in exon 6 (rs160146162 and rs160146160; encoding the FERM domain) and one polymorphic site in exon 24 of the gene (rs160146116; JH1 kinase domain) were genotyped. In our study, the associations examined for cattle were inconclusive. However, Hereford and Limousin cattle with genotypes (e16/I) and (e23/III) tended to have the highest body weight and better daily gains ( ≤ 0.05). No clear tendency was observed in the selected reproductive traits. In the case of sheep, regardless of breed, individuals with the (e6/I), (e6/seq), and (e24/188III) genotypes had the highest body weights and daily gains in the study periods ( ≤ 0.01). The same individuals in the Pomeranian breed also had better fertility and lamb survival ( ≤ 0.01). To the best of our knowledge, these are the first association studies for all these polymorphic sites. Single-nucleotide polymorphisms in the gene can serve as genetic markers for growth and selected reproductive traits in ruminants given that they are further investigated in subsequent populations and analyzed using haplotype and/or combined genotype systems.
Janus激酶2（JAK2）酪氨酸激酶是II类细胞因子受体信号转导的重要组成部分，包括生长激素受体。因此，它可能在促生长轴的信号通路中发挥关键作用，该信号通路影响反刍动物的生长、发育和繁殖性状。为此，对三个品种的牛（海福特牛、安格斯牛和利木赞牛；共781头），分析了位于第16外显子（rs210148032；p.Ile704Val，位于假激酶（JH2）内）和第23外显子（沉默突变rs211067160，位于JH1激酶结构域内）的两个多态性位点。对两个品种的绵羊（博美羊和萨福克羊；共333只），对第6外显子的两个多态性位点（rs160146162和rs160146160；编码FERM结构域）和该基因第24外显子的一个多态性位点（rs160146116；JH1激酶结构域）进行了基因分型。在我们的研究中，对牛所检测的关联尚无定论。然而，基因型为（e16/I）和（e23/III）的海福特牛和利木赞牛往往具有最高的体重和更好的日增重（P≤0.05）。在所选择的繁殖性状中未观察到明显趋势。在绵羊中，无论品种如何，基因型为（e6/I）、（e6/seq）和（e&4/188III）的个体在研究期间具有最高的体重和日增重（P≤0.01）。博美羊品种中的相同个体也具有更好的繁殖力和羔羊存活率（P≤0.01）。据我们所知，这些是所有这些多态性位点的首次关联研究。考虑到在后续群体中对其进行进一步研究并使用单倍型和/或组合基因型系统进行分析，该基因中的单核苷酸多态性可作为反刍动物生长和所选繁殖性状的遗传标记。
