LAQV-Requimte, Department of Chemistry, NOVA School of Science and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal.
ITQB NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, 2780-157 Oeiras, Portugal.
Molecules. 2023 Jul 25;28(15):5621. doi: 10.3390/molecules28155621.
Arabinoxylan has prebiotic properties, as it is able to resist digestion in the small intestine and undergoes fermentation in the large intestine. In this work, arabinoxylan was extracted from corn fiber using an alkaline solution and further purified with membrane processing. It was found that the extracts were mainly composed of xylose (50-52%), arabinose (37-39%), galactose (9%) and glucose (1-4%), with an A/X ratio of 0.72-0.77. All the extracts were composed of phenolic compounds, including ferulic acid derivatives such as dimers, trimers and tetramers. The purified extract had a lower concentration of ferulic and -coumaric acid (0.004 and 0.02 mg/mg, respectively) when compared to raw extract (19.30 and 2.74 mg/mg, respectively). The same effect was observed for the antioxidant activity, with purified extracts having a lower value (0.17 ± 0.02 µmol TEAC/mg) when compared to the raw extract (2.20 ± 0.35 µmol TEAC/mg). The purified extract showed a greater antiproliferative effect against the HT29 cell line with EC = 0.12 ± 0.02 mg/mL when compared to the raw extract (EC = 5.60 ± 1.6 mg/mL). Both raw and purified extracts did not show any cytotoxicity to the Caco-2 cell line in the maximum concentration tested (10 mg/mL).
阿拉伯木聚糖具有益生元特性，因为它能够抵抗小肠中的消化，并在大肠中发酵。在这项工作中，使用碱性溶液从玉米纤维中提取阿拉伯木聚糖，然后用膜处理进一步纯化。结果发现，提取物主要由木糖（50-52%）、阿拉伯糖（37-39%）、半乳糖（9%）和葡萄糖（1-4%）组成，A/X 比为 0.72-0.77。所有提取物均由酚类化合物组成，包括阿魏酸衍生物，如二聚体、三聚体和四聚体。与粗提取物（分别为 19.30 和 2.74 mg/mg）相比，纯化提取物中的阿魏酸和 -香豆酸浓度较低（分别为 0.004 和 0.02 mg/mg）。抗氧化活性也观察到相同的效果，纯化提取物的值较低（0.17 ± 0.02 µmol TEAC/mg），而粗提取物的值较高（2.20 ± 0.35 µmol TEAC/mg）。与粗提取物（EC = 5.60 ± 1.6 mg/mL）相比，纯化提取物对 HT29 细胞系的增殖抑制作用更强，EC = 0.12 ± 0.02 mg/mL。在测试的最大浓度（10 mg/mL）下，粗提取物和纯化提取物对 Caco-2 细胞系均无任何细胞毒性。
