Slovenian Forestry Institute, Večna pot 2, SI-1000 Ljubljana, Slovenia.
Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia.
Molecules. 2023 Jul 29;28(15):5738. doi: 10.3390/molecules28155738.
The grains of three barley varieties were milled and sieved to obtain respective milling fractions with a content of beta-glucans (b-G) from 1.4 to 10.7%. The enriched fraction obtained by the extraction and precipitation contained 24.7% of b-G. The differences between the ratio of stable C carbon isotopes were established. Milling fractions with coarse particles had more beta-glucans and a more negative ratio of δC isotope in comparison to the respective intact barley grain. However, the enriched fraction had a less negative isotope ratio. So, it is not expected that the deviation from the stable isotope ratio of grain in milling fractions is the result of the content of b-G, but it depends on other barley grain constituents. In different parts of barley grain, there are substances with different stable isotope ratios, and by milling and sieving, they are assorted to the same milling fraction with most of the b-G. The method for determining the ratio of a stable carbon isotope in diverse barley grain fractions, applied in this investigation, is potentially opening the possibility for an additional method of screening the concentration of bioactive constituents in barley grain.
三种大麦品种的麦粒经粉碎和筛分后，得到β-葡聚糖（b-G）含量为 1.4-10.7%的相应制粉级分。通过提取和沉淀获得的富集级分含有 24.7%的 b-G。建立了稳定碳同位素比值的差异。与完整的大麦籽粒相比，粗颗粒的制粉级分具有更多的β-葡聚糖和更负的δC 同位素比值。然而，富集级分的同位素比值则不那么负。因此，制粉级分中谷物稳定同位素比值的偏差预计不是 b-G 含量的结果，而是取决于其他大麦籽粒成分。在大麦籽粒的不同部位，存在具有不同稳定同位素比值的物质，通过粉碎和筛分，它们被分到含有大部分 b-G 的相同制粉级分中。本研究中应用的测定不同大麦籽粒级分中稳定碳同位素比值的方法，为筛选大麦籽粒中生物活性成分的浓度提供了一种潜在的附加方法。
