CBQF Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
Molecules. 2023 Aug 7;28(15):5923. doi: 10.3390/molecules28155923.
Nowadays, with consumers' requirements shifting towards more natural solutions and the advent of nutraceutical-based approaches, new alternatives for obesity management are being developed. This work aimed to show, for the first time, the potential of avocado oil-fortified cheese as a viable foodstuff for obesity management through complex in vitro cellular models. The results showed that oleic and palmitic acids' permeability through the Caco-2/HT29-MTX membrane peaked at the 2h mark, with the highest apparent permeability being registered for oleic acid (0.14 cm/s). Additionally, the permeated compounds were capable of modulating the metabolism of adipocytes present in the basal compartment, significantly reducing adipokine (leptin) and cytokine (MPC-1, IL-10, and TNF-α) production. The permeates (containing 3.30 µg/mL of palmitic acid and 2.16 µg/mL of oleic acid) also presented an overall anti-inflammatory activity upon Raw 264.7 macrophages, reducing IL-6 and TNF-α secretion. Despite in vivo assays being required, the data showed the potential of a functional dairy product as a valid food matrix to aid in obesity management.
如今，随着消费者对更自然解决方案的需求转变以及基于营养保健品的方法的出现，正在开发新的肥胖管理替代方案。本研究首次旨在通过复杂的体外细胞模型展示鳄梨油强化奶酪作为肥胖管理可行食品的潜力。结果表明，油酸和棕榈酸通过 Caco-2/HT29-MTX 膜的通透性在 2 小时标记处达到峰值，油酸的表观通透性最高（0.14 cm/s）。此外，渗透的化合物能够调节基础隔室中存在的脂肪细胞的代谢，显著降低脂肪因子（瘦素）和细胞因子（MPC-1、IL-10 和 TNF-α）的产生。渗透物（含有 3.30 µg/mL 的棕榈酸和 2.16 µg/mL 的油酸）对 Raw 264.7 巨噬细胞也具有整体抗炎活性，减少 IL-6 和 TNF-α 的分泌。尽管需要进行体内试验，但数据显示了功能性乳制品作为辅助肥胖管理的有效食品基质的潜力。
