Popoola Olugbenga K, Marnewick Jeanine L, Iwuoha Emmanuel I, Hussein Ahmed A
Applied Microbial and Health Biotechnology Institute, Cape Peninsula University of Technology, Bellville 7535, South Africa.
Chemistry Department, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa.
Plants (Basel). 2023 Aug 4;12(15):2870. doi: 10.3390/plants12152870.
Chromatographic fractionation of a methanol extract of afforded seven known compounds. The isolated compounds were identified as 5,7,8-trihydroxy-3,6-dimethoxyflavone-8--2-methyl-2-butanoate (C-), 5,7-dihydroxy-3,6,8-trimethoxyflavone (C-), 5-hydroxy-3,6,7,8-tetramethoxyflavone (C-), 5-hydroxy-3,6,7-trimethoxyflavone (C-), -kaurenoic acid (C-), -kauran-18-al (C-), and 15-α-hydroxy-(-)--kaur-16-en-19-oic acid (C-). Compounds C--C- demonstrated high antioxidant capacities on ORAC hydroxyl radical (2.114 ± 4.01; 2.413 ± 6.20; 1.924 ± 16.40; 1.917 ± 3.91) × 10; ORAC peroxyl radical (3.523 ± 3.22; 2.935 ± 0.13; 2.431 ± 8.63; 2.814 ± 5.20) × 10 µMTE/g; and FRAP (1251.45 ± 4.18; 1402.62 ± 5.77) µMAAE/g, respectively. Moderate inhibitory activities against Fe-induced lipid peroxidation were observed for C-C as IC values of 13.123 ± 0.34, 16.421 ± 0.92, 11.64 ± 1.72, 14.90 ± 0.06 µg/mL, respectively, while their respective anti-tyrosinase activities with IC values of 25.735 ± 9.62, 24.062 ± 0.61, 39.03 ± 13.12, 37.67 ± 0.98 µg/mL were also observed. All compounds demonstrated TEAC values within the range of 1105-1424 µMTE/g. The result is an indication that a methanol extract of might possibly be a good source of natural antioxidants against ailments caused by cellular oxidative stress and as inhibitors against skin depigmentation, as well as possible raw materials needed for slowing down perishable agricultural products. This is the first report on the phytochemical and biological evaluation of .
对[提取物名称未给出]的甲醇提取物进行色谱分离得到了七种已知化合物。分离出的化合物被鉴定为5,7,8 - 三羟基 - 3,6 - 二甲氧基黄酮 - 8 - O - 2 - 甲基 - 2 - 丁酸酯（C - ）、5,7 - 二羟基 - 3,6,8 - 三甲氧基黄酮（C - ）、5 - 羟基 - 3,6,7,8 - 四甲氧基黄酮（C - ）、5 - 羟基 - 3,6,7 - 三甲氧基黄酮（C - ）、β - 贝壳杉烯酸（C - ）、β - 贝壳杉 - 18 - 醛（C - ）和15 - α - 羟基 - （ - ） - 贝壳杉 - 16 - 烯 - 19 - 酸（C - ）。化合物C - - C - 在ORAC羟基自由基清除能力方面表现出高抗氧化能力（(2.114 ± 4.01; 2.413 ± 6.20; 1.924 ± 16.40; 1.917 ± 3.91) × 10；ORAC过氧自由基清除能力为(3.523 ± 3.22; 2.935 ± 0.13; 2.431 ± 8.63; 2.814 ± 5.20) × 10 μMTE/g；FRAP值分别为(1251.45 ± 4.18; 1402.62 ± 5.77) μMAAE/g）。观察到C - C对铁诱导的脂质过氧化具有中等抑制活性，其IC值分别为13.123 ± 0.34、16.421 ± 0.92、11.64 ± 1.72、14.90 ± 0.06 μg/mL，同时还观察到它们各自的抗酪氨酸酶活性，IC值分别为25.735 ± 9.62、24.062 ± 0.61、39.03 ± 13.12、37.67 ± 0.98 μg/mL。所有化合物的TEAC值在1105 - 1424 μMTE/g范围内。该结果表明，[提取物名称未给出]的甲醇提取物可能是对抗细胞氧化应激引起的疾病的天然抗氧化剂的良好来源，也是皮肤色素沉着抑制剂以及减缓易腐农产品变质所需的可能原材料。这是关于[提取物名称未给出]的植物化学和生物学评价的首次报道。
