Mouafo Hippolyte Tene, Tchuenchieu Alex Dimitri Kamgain, Nguedjo Maxwell Wandji, Edoun Ferdinand Lanvin Ebouel, Tchuente Boris Ronald Tonou, Medoua Gabriel Nama
Centre for Food and Nutrition Research, Institute of Medical Research and Medicinal Plants Studies, POBOX 13033, Yaoundé Cameroon.
Department of Biochemistry, Faculty of Sciences, University of Yaoundé 1, POBOX 812, Yaoundé Cameroon.
Heliyon. 2021 Apr 19;7(4):e06830. doi: 10.1016/j.heliyon.2021.e06830. eCollection 2021 Apr.
This study aimed at evaluating the antimicrobial potential of aqueous, ethanolic and methanolic extracts of two Cameroonian plants against selected foodborne pathogens. Bioactive compounds were extracted from De Wild seeds and Banks ex. C. F. Gaertn leaves using distilled water, ethanol and methanol as solvents. The extracts were tested against O157, , , , , , , and using the microdilution method. The results showed that distilled water extracted a more important mass of phytochemical compounds (18.0-24.60%) compared to ethanol (4.80-5.0%) and methanol (4.20-4.60%). All the extracts exhibited significant antimicrobial activity with MIC values ranging from 5 to 20 μg/mL for seeds extracts and from 1.0 to 20 μg/mL for leaves extracts. The different plant extracts were ten times less active than gentamicin. The most active extracts were obtained using ethanol as solvent and was the most resistant pathogen to all extracts (MBC>20 μg/mL). extracts were bactericidal against and while the reference antibiotic (gentamicin) was bacteriostatic against these pathogens. The results obtained from this study suggest the studied local plant materials as a source of antimicrobial compounds which can be valorized in the medical field as substitute of antibiotics for which many microorganisms have nowadays developed resistance mechanisms. Further studies need to be performed in order to characterize and identify these antimicrobial active molecules.
本研究旨在评估两种喀麦隆植物的水提取物、乙醇提取物和甲醇提取物对选定食源性病原体的抗菌潜力。使用蒸馏水、乙醇和甲醇作为溶剂，从德维尔德种子和班克斯（以C.F.盖尔特纳命名）的叶子中提取生物活性化合物。采用微量稀释法对提取物进行针对O157、[此处原文部分病原体名称缺失]的测试。结果表明，与乙醇（4.80 - 5.0%）和甲醇（4.20 - 4.60%）相比，蒸馏水提取的植物化学成分质量更高（18.0 - 24.60%）。所有提取物均表现出显著的抗菌活性，种子提取物的最低抑菌浓度（MIC）值为5至20μg/mL，叶子提取物的MIC值为1.0至20μg/mL。不同植物提取物的活性比庆大霉素低十倍。以乙醇为溶剂获得的提取物活性最高，[此处原文部分病原体名称缺失]对所有提取物的抗性最强（最低杀菌浓度>20μg/mL）。[此处原文部分提取物和病原体名称缺失]提取物对[此处原文部分病原体名称缺失]具有杀菌作用，而参考抗生素（庆大霉素）对这些病原体具有抑菌作用。本研究结果表明，所研究的当地植物材料可作为抗菌化合物的来源，在医学领域可作为抗生素的替代品加以利用，如今许多微生物已对这些抗生素产生了耐药机制。需要进一步开展研究以表征和鉴定这些抗菌活性分子。
