A choline-devoid diet, carcinogenic in the rat, induces DNA damage and repair.

A diet deficient in choline when fed to rats for as few as 3 days resulted in liver DNA damage. The damage could be monitored as alkali-labile lesions using alkaline-sucrose gradients as well as alkaline elution technique. The DNA damage disappeared when the choline-deficient diet was replaced by a choline-supplemented diet suggesting the alkali-labile lesions were repairable. The DNA damage was detected at a time when no liver cell death was detectable. The induction of DNA damage in a proliferating liver by the choline-deficient diet may be an early important event leading to initiation of liver carcinogenesis.