Nanoimaging Core Facility at the University of Nebraska Medical Center, Omaha, NE, USA.
Department of Chemistry, Nanoscale Science Program, University of North Carolina, Charlotte, NC, USA.
Methods Mol Biol. 2023;2709:191-202. doi: 10.1007/978-1-0716-3417-2_12.
The protocol described in this chapter allows for acquiring topography images of RNA-based nanoring structures and assessing their dynamic properties using atomic force microscopy (AFM) imaging. AFM is an indispensable tool for characterization of nucleic acid-based nanostructures with the exceptional capability of observing complexes in the range of a few nanometers. This method can visualize structural characteristics and evaluate differences between individual structurally different RNA nanorings. Due to the highly resolved AFM topography images, we introduce an approach that allows to distinguish the differences in the dynamic behavior of RNA nanoparticles not amenable to other experimental techniques. This protocol describes in detail the preparation procedures of RNA nanostructures, AFM imaging, and data analysis.
本章中描述的方案允许使用原子力显微镜（AFM）成像技术获取基于 RNA 的纳米环结构的形貌图像，并评估其动态特性。AFM 是一种不可或缺的工具，可用于对基于核酸的纳米结构进行特性分析，具有观察几纳米范围内复合物的独特能力。该方法可以可视化结构特征，并评估不同结构的 RNA 纳米环之间的差异。由于 AFM 形貌图像具有高分辨率，我们引入了一种方法，可以区分其他实验技术难以区分的 RNA 纳米颗粒的动态行为差异。本方案详细描述了 RNA 纳米结构的制备程序、AFM 成像和数据分析。
