将功能性RNA环反向转染至癌细胞后进行体外照射。
Reverse Transfection of Functional RNA Rings into Cancer Cells Followed by in Vitro Irradiation.
作者信息
de Freitas Saito Renata, Ferreira Isabella Nevoni, Rangel Maria Cristina, Chammas Roger
机构信息
Comprehensive Center for Precision Oncology, Centro de Investigação Translacional em Oncologia (LIM24), Departamento de Radiologia e Oncologia, Faculdade de Medicina da Universidade de São Paulo and Instituto do Câncer do Estado de São Paulo, São Paulo, SP, Brazil.
出版信息
Methods Mol Biol. 2023;2709:263-276. doi: 10.1007/978-1-0716-3417-2_18.
RNA nanoparticles are promising therapeutic platforms to improve radiotherapy since they can be functionalized with multiple small interfering RNAs (RNAi) to simultaneously silence critical radioresistance genes. Here we describe the transfer of RNA rings to mammalian cancer cells through reverse transfection, followed by in vitro irradiation and biological assays as surrogates' endpoints for radiotherapy efficacy.
RNA纳米颗粒有望成为改善放射治疗的治疗平台，因为它们可以用多种小干扰RNA（RNAi）进行功能化，以同时沉默关键的抗辐射基因。在这里，我们描述了通过反向转染将RNA环转移到哺乳动物癌细胞中，随后进行体外照射和生物学检测，作为放射治疗疗效的替代终点。
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