Afonin Kirill A, Viard Mathias, Koyfman Alexey Y, Martins Angelica N, Kasprzak Wojciech K, Panigaj Martin, Desai Ravi, Santhanam Arti, Grabow Wade W, Jaeger Luc, Heldman Eliahu, Reiser Jakob, Chiu Wah, Freed Eric O, Shapiro Bruce A
Basic Research Laboratory, Center for Cancer Research, National Cancer Institute , Frederick, Maryland 21702, United States.
Nano Lett. 2014 Oct 8;14(10):5662-71. doi: 10.1021/nl502385k. Epub 2014 Sep 30.
Our recent advancements in RNA nanotechnology introduced novel nanoscaffolds (nanorings); however, the potential of their use for biomedical applications was never fully revealed. As presented here, besides functionalization with multiple different short interfering RNAs for combinatorial RNA interference (e.g., against multiple HIV-1 genes), nanorings also allow simultaneous embedment of assorted RNA aptamers, fluorescent dyes, proteins, as well as recently developed RNA-DNA hybrids aimed to conditionally activate multiple split functionalities inside cells.
我们在RNA纳米技术方面的最新进展引入了新型纳米支架（纳米环）；然而，其在生物医学应用中的潜力从未得到充分展现。如本文所述，除了用多种不同的短干扰RNA进行功能化以实现组合式RNA干扰（例如，针对多种HIV-1基因）外，纳米环还允许同时嵌入各种RNA适配体、荧光染料、蛋白质，以及最近开发的旨在在细胞内有条件地激活多种分裂功能的RNA-DNA杂交体。
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